Magesh Kuppan, Singh Sukhvant, Wu Shu Pao, Velmathi Sivan
Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India.
Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 30010, Republic of China.
Anal Methods. 2023 Aug 24;15(33):4159-4167. doi: 10.1039/d3ay00987d.
Only a few probes are suited for highly acidic environments and sensitive to pH values below 4. Thus, finding a solution for detecting strong acidic (pH value below 2) conditions is still challenging. Herein, we constructed and created a pH-switched fluorescent probe based on pyrene and a heteroatom containing pyridine unit. When exposed to acidic environments (pH 2.0), the probe's fluorescence redshifted with distinct colour and fluorescence changes owing to protonation on the nitrogen atom containing pyridine moiety, which could be deprotonated by HS selectively compared to other competing analytes. Pyr can detect HS with a rapid response within 5 s and showed very good quantum yield under acidic environments. The sensing mechanism was confirmed by Density Functional Theory (DFT) studies using the B3LYP and 6-31G+ (d) basis sets. Furthermore, the probe was utilized to monitor HS in actual water samples and identify HS gas by a simple paper strip test.
只有少数探针适用于高酸性环境且对pH值低于4敏感。因此，找到检测强酸性（pH值低于2）条件的解决方案仍然具有挑战性。在此，我们构建并创建了一种基于芘和含杂原子吡啶单元的pH开关荧光探针。当暴露于酸性环境（pH 2.0）时，由于含吡啶部分的氮原子质子化，探针的荧光发生红移，伴有明显的颜色和荧光变化，与其他竞争性分析物相比，HS可选择性地使其去质子化。Pyr能在5秒内快速响应检测HS，且在酸性环境下显示出非常好的量子产率。通过使用B3LYP和6-31G+（d）基组的密度泛函理论（DFT）研究证实了传感机制。此外，该探针被用于监测实际水样中的HS，并通过简单的纸条测试识别HS气体。
