Liaoning Key Laboratory of Development and Utilization for Natural Products Active Molecules, School of Chemistry and Life Science, Anshan Normal University, Anshan, China.
College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, Jilin University, Qianjin Street 2699, Changchun, China.
Luminescence. 2023 Feb;38(2):159-165. doi: 10.1002/bio.4435. Epub 2023 Jan 10.
Hydrazine (N H ) is a highly toxic and harmful chemical reagent. Fluorescent probes are simple and efficient tools for sensitive monitoring of N H enrichment in the environment, humans, animals, and plants. In this work, a ratiometric fluorescent probe (FP-1) containing coumarin was used for hydrazine detection. The proposed FP-1 probe had a linear detection range of 0-250 μM and a limit of detection (LOD) of 0.059 μM (1.89 ppb). A large red Stokes shift was observed in fluorescence and UV-vis absorption spectra due to the hydrolysis of ester bonds between FP-1 and hydrazine. The hydrazine detection mechanism of FP-1 was also investigated using density functional theory (DFT) calculations. Finally, FP-1 could sensitively and selectively monitor hydrazine in actual water samples and BEAS-2B cells. Therefore, it has great application potential in environmental monitoring and disease diagnosis.
水合肼（N₂H₄）是一种高毒性和有害的化学试剂。荧光探针是用于灵敏监测环境、人类、动物和植物中 N₂H₄富集的简单而有效的工具。在这项工作中，使用了一种含有香豆素的比率型荧光探针（FP-1）来检测水合肼。所提出的 FP-1 探针具有 0-250 μM 的线性检测范围和 0.059 μM（1.89 ppb）的检测限。由于 FP-1 与水合肼之间酯键的水解，在荧光和紫外可见吸收光谱中观察到较大的红移斯托克斯位移。还使用密度泛函理论（DFT）计算研究了 FP-1 的水合肼检测机制。最后，FP-1 可以在实际水样和 BEAS-2B 细胞中灵敏且选择性地监测水合肼。因此，它在环境监测和疾病诊断方面具有巨大的应用潜力。
