Institute of Smart Biomedical Materials, School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, P.R. China.
Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou, P.R. China.
Tissue Eng Part C Methods. 2023 Nov;29(11):535-544. doi: 10.1089/ten.TEC.2023.0132. Epub 2023 Sep 29.
The management of large amounts of bio-wastes, such as bovine femurs from kitchens and slaughterhouses, has long been a challenging issue. However, through the utilization of a hydrothermal process, it is possible to transform these bio-wastes into valuable products. In this study, we focused on extracting hydroxyapatite (HAp), the primary inorganic component of bovine femurs, for potential use in bone tissue engineering scaffolds. By subjecting the femurs to hydrothermal treatment at varying times and solvents, we successfully decomposed and removed the organic matter present, resulting in the extraction of HAp. To comprehensively evaluate the properties of the extracted HAp, we employed several characterization techniques that provided valuable insights into the structure, morphology, and elemental composition of the extracted HAp. Furthermore, we conducted a Cell Counting Kit-8 assay, which confirmed the favorable biocompatibility of the extracted HAp. Overall, this study highlights the potential of hydrothermal treatment as an environmentally friendly and cost-effective method for handling bio-waste, specifically bovine femurs. The extracted HAp exhibits promising characteristics, making it suitable for a wide range of biomedical applications. This research contributes to the sustainable utilization of bio-waste and underscores the importance of resourceful exploitation for environmental protection.
大量生物废物的管理一直是一个具有挑战性的问题，例如厨房和屠宰场的牛股骨。然而，通过水热法的利用，将这些生物废物转化为有价值的产品成为可能。在这项研究中，我们专注于提取羟磷灰石（HAp），这是牛股骨的主要无机成分，用于潜在的骨组织工程支架。通过对股骨进行不同时间和溶剂的水热处理，我们成功地分解并去除了存在的有机物，从而提取了 HAp。为了全面评估提取的 HAp 的性质，我们采用了几种表征技术，这些技术提供了有关提取的 HAp 的结构、形态和元素组成的有价值的见解。此外，我们进行了细胞计数试剂盒-8 测定，该测定证实了提取的 HAp 的良好生物相容性。总的来说，这项研究强调了水热处理作为一种环保且具有成本效益的方法来处理生物废物，特别是牛股骨的潜力。提取的 HAp 表现出有希望的特性，使其适合广泛的生物医学应用。这项研究有助于生物废物的可持续利用，并强调了资源开发对环境保护的重要性。
