1 Department of Polymer Chemistry and Technology, Kaunas University of Technology, Kaunas, Lithuania.
2 Department of Chemical Engineering, Izmir Institute of Technology, Izmir, Turkey.
J Biomater Appl. 2019 Jan;33(6):876-890. doi: 10.1177/0885328218811040. Epub 2018 Nov 19.
Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility. Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine. Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications. In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration. Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount. Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen. Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds. Interconnected highly porous structure of the scaffolds was confirmed by micro-computed tomography. The mean pore size of the scaffolds was 510 µm with a porosity of 85%. The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds. In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds. In addition, cuttlebone-derived hydroxyapatite significantly induced the ALP activity and osteocalcin secretion. Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
最近，由于其具有生物相容性、低免疫原性和广泛易得性等有前景的特性，海洋衍生材料在生物医学领域的应用引起了人们的关注。在这些海洋来源中，乌贼骨已被用作具有痕量元素组成的传统药物中的有价值成分。最近的研究集中在将乌贼骨用作组织工程应用的生物活性剂。在这项研究中，通过水热合成乌贼骨获得了羟基磷灰石颗粒，并将其掺入纤维素支架中，以制造用于骨再生的骨传导复合支架。对来自不同沿海地区的原始乌贼骨材料和乌贼骨衍生的 HAp 的元素分析表明，各种宏量、微量和痕量元素 - Ca、P、Na、Mg、Cu、Sr、Cl、K、S、Br、Fe 和 Zn 的含量非常相似。此外，在任何乌贼骨标本中都没有检测到生物上不利的重金属，如 Ag、Cd、Pb 或 V。通过水热处理进一步从乌贼骨微球中合成碳酸羟基磷灰石颗粒，并将其用作纤维素基复合支架制备的矿物填料。通过微计算机断层扫描证实了支架的互连高多孔结构。支架的平均孔径为 510 µm，孔隙率为 85%。通过压缩试验对支架进行了机械特性表征，并且掺入乌贼骨衍生的 HAp 增强了纤维素支架的机械性能。体外细胞培养研究表明，MG-63 细胞在支架上增殖良好。此外，乌贼骨衍生的羟基磷灰石显著诱导碱性磷酸酶活性和骨钙素分泌。此外，HAp 掺入增加了表面矿化，这是骨组织再生的主要步骤。
