Department of Anaesthesiology, West China Hospital, Sichuan University, China.
Laboratory of Anaesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anaesthesiology, West China Hospital, Sichuan University, China.
Anal Methods. 2023 Aug 24;15(33):4179-4186. doi: 10.1039/d3ay01168b.
Ciprofol (HSK 3486, CHO), a novel 2,6-disubstituted phenol derivative similar to propofol, is a new type of intravenous general anaesthetic. We found that the exhaled ciprofol concentration could be measured online by ultraviolet time-of-flight mass spectrometry (UV-TOFMS), which could be used to predict the plasma concentration and anaesthetic effects of ciprofol. In this study, we present the calibration method and validation results of UV-TOFMS for the quantification of ciprofol gas. Using a self-developed gas generator to prepare different concentrations of ciprofol calibration gas, we found a linear correlation between the concentration and intensity of ciprofol from 0 parts per trillion by level (pptv) to 485.85 pptv ( = 0.9987). The limit of quantification was 48.59 pptv and the limit of detection was 7.83 pptv. The imprecision was 12.44% at 97.17 pptv and was 8.96% at 485.85 pptv. The carry-over duration was 120 seconds. In addition, we performed a continuous infusion of ciprofol in beagles, measured the exhaled concentration of ciprofol by UV-TOFMS, determined the plasma concentration by high-performance liquid chromatography, and monitored the anaesthetic effects as reflected by the bispectral index value. The results showed that the exhaled and plasma concentrations of ciprofol were linearly correlated. The exhaled ciprofol concentration correlated well with the anaesthetic effect. The study showed that we could use UV-TOFMS to provide a continuous measurement of gaseous ciprofol concentration at 20 second intervals.
西普罗夫（HSK3486，CHO）是一种新型的 2,6-二取代酚类衍生物，类似于丙泊酚，是一种新型的静脉全身麻醉剂。我们发现，呼出的西普罗夫浓度可以通过紫外飞行时间质谱（UV-TOFMS）在线测量，这可以用来预测西普罗夫的血浆浓度和麻醉效果。在这项研究中，我们介绍了 UV-TOFMS 定量检测西普罗夫气体的校准方法和验证结果。我们使用自行开发的气体发生器来制备不同浓度的西普罗夫校准气体，发现西普罗夫的浓度与强度之间存在线性关系，从 0 个万亿分之一（pptv）到 485.85 pptv（=0.9987）。定量下限为 48.59 pptv，检测下限为 7.83 pptv。在 97.17 pptv 时的精密度为 12.44%，在 485.85 pptv 时的精密度为 8.96%。无拖带时间为 120 秒。此外，我们在比格犬中进行了西普罗夫的连续输注，通过 UV-TOFMS 测量呼出的西普罗夫浓度，通过高效液相色谱法测定血浆浓度，并通过双谱指数值监测麻醉效果。结果表明，呼出和血浆中的西普罗夫浓度呈线性相关。呼出的西普罗夫浓度与麻醉效果密切相关。该研究表明，我们可以使用 UV-TOFMS 以 20 秒的间隔提供连续测量气态西普罗夫浓度。
