McKee M J, Knowles C O
Ecotoxicol Environ Saf. 1986 Aug;12(1):70-84. doi: 10.1016/0147-6513(86)90007-2.
Daphnia magna were exposed to fenvalerate at nominal concentrations of 0.5, 0.25, 0.13, 0.06, and 0.03 micrograms/liter for 21 days. On Days 7 and 21 of exposure, levels of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), adenosine diphosphate (ADP), adenosine triphosphate (ATP), glycogen, and lipid were measured, and the results were related to survival and reproduction during the 21-day test period. Survival was not significantly (alpha = 0.05) affected by the 21-day exposure; however, reproduction was reduced at fenvalerate concentrations of 0.25 and 0.5 micrograms/liter. On Day 7, protein, RNA, ADP, caloric equivalents, and glycogen were also significantly reduced at fenvalerate concentrations of 0.25 and 0.5 micrograms/liter. Thus, these biochemical parameters identified the same no observable effect concentration (NOEC) as did reproduction. Variables derived from biochemical parameters were related to reduced protein growth and reproduction. Decreases in protein/RNA and glycogen/lipid ratios and increases in ADP/DNA, ADP/ATP, protein/RNA/DNA, and lipid/DNA ratios were observed at Day 7 for those daphnia exposed to 0.5 micrograms/liter of fenvalerate. The only derived variable that reflected the reduced protein growth at Day 7 in daphnia exposed to 0.25 micrograms/liter of fenvalerate was the glycogen/lipid ratio. Biochemical determinations at Day 21 indicated that the organisms exposed to 0.25 micrograms/liter of fenvalerate were not different from controls, whereas those exposed to 0.5 micrograms/liter were still affected.
将大型溞暴露于浓度分别为0.5、0.25、0.13、0.06和0.03微克/升的氰戊菊酯中21天。在暴露的第7天和第21天，测量核糖核酸（RNA）、脱氧核糖核酸（DNA）、二磷酸腺苷（ADP）、三磷酸腺苷（ATP）、糖原和脂质的水平，并将结果与21天试验期内的存活和繁殖情况相关联。21天的暴露对存活率没有显著影响（α = 0.05）；然而，在氰戊菊酯浓度为0.25和0.5微克/升时，繁殖率降低。在第7天，氰戊菊酯浓度为0.25和0.5微克/升时，蛋白质、RNA、ADP、热量当量和糖原也显著降低。因此，这些生化参数确定的无可见效应浓度（NOEC）与繁殖情况相同。从生化参数得出的变量与蛋白质生长和繁殖的降低有关。对于暴露于0.
