Department of Pediatrics, The Third Xiangya Hospital Central South University, Changsha, Hunan, China.
Methods Mol Biol. 2023;2712:149-156. doi: 10.1007/978-1-0716-3433-2_13.
Ferroptosis is a form of regulated cell death that occurs due to abnormal lipid metabolism. Lipids, which have been identified in over 45,000 different molecular species, play essential roles in modulating basic life processes. The process of ferroptosis is highly reliant on various lipid species, with polyunsaturated fatty acids (PUFAs) playing a central role in driving this process. Recent advances in mass spectrometry-based lipidomics have led to a surge in studies on ferroptosis. To explore the mechanism of lipid homeostasis in ferroptosis, the development of lipidomics techniques is critical. Currently, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) are the most widely used analytical techniques in lipidomics. These techniques offer deeper insights into the complex lipid mechanisms that underlie ferroptosis.
铁死亡是一种由于脂质代谢异常导致的细胞程序性死亡形式。脂质在超过 45000 种不同的分子物种中被鉴定出来，在调节基本生命过程中发挥着至关重要的作用。铁死亡过程高度依赖于各种脂质物种，其中多不饱和脂肪酸（PUFAs）在驱动这一过程中起着核心作用。基于质谱的脂质组学的最新进展导致了铁死亡研究的激增。为了探索铁死亡中脂质动态平衡的机制，脂质组学技术的发展至关重要。目前，液相色谱-串联质谱（LC-MS/MS）和气相色谱-质谱（GC-MS）是脂质组学中最广泛使用的分析技术。这些技术提供了对铁死亡背后复杂脂质机制的更深入了解。
