Department of Neurological Surgery and The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA.
Methods Mol Biol. 2023;2696:223-238. doi: 10.1007/978-1-0716-3350-2_15.
The inflammasome is a multiprotein complex that is responsible for mounting an innate immune response through the activation of caspase-1 and the cleavage of interleukin-1β. This multiprotein complex plays an important role in a variety of central nervous system (CNS) diseases and conditions such as Alzheimer's disease, Parkinson's disease, multiple sclerosis, stroke, and traumatic brain injury, among others. Here we describe methodological procedures to carry out immunoblotting and immunohistochemical techniques used to study inflammasome signaling in CNS tissues (brain and spinal cord).
炎症小体是一种多蛋白复合物，通过激活 caspase-1 和白介素-1β的切割，负责引发先天免疫反应。这种多蛋白复合物在多种中枢神经系统 (CNS) 疾病和病症中发挥重要作用，如阿尔茨海默病、帕金森病、多发性硬化症、中风和创伤性脑损伤等。在这里，我们描述了用于研究 CNS 组织（大脑和脊髓）中炎症小体信号的免疫印迹和免疫组织化学技术的方法程序。
