School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 12 Zhangzhou Road, Zibo 255049, Shandong Province, China.
State Key Laboratory for Modification of Chemical Fiber and Polymer Materials, Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, China.
Food Chem. 2024 Jan 15;431:137067. doi: 10.1016/j.foodchem.2023.137067. Epub 2023 Aug 12.
A novel dual-signal fluorescent sensor was developed for detecting organophosphorus pesticides (OPs). It relies on the catalytic activities of acetylcholinesterase (AChE) and choline oxidase (ChOx) to generate hydrogen peroxide (HO) through the conversion of acetylcholine (ACh) to choline·HO then oxidizes ferrocene-modified tetraphenylethylene (TPE-Fc) to its oxidized state (TPE-Fc), resulting in enhanced cyan fluorescence due to aggregation. Simultaneously, ferrocene oxidation generates hydroxyl radicals (•OH), causing a decrease in orange fluorescence of glutathione-synthesized gold nanoclusters (GSH-AuNCs). The presence of OPs restricts AChE activity, reducing HO production. Increasing OPs concentration leads to decreased cyan fluorescence and increased orange fluorescence, enabling visual OPs detection. The sensor has a linear dynamic range of 10-2000 ng/mL with a detection limit of 2.05 ng/mL. Smartphone-based color identification and a WeChat mini program were utilized for rapid OPs analysis with successful outcomes.
一种新型的双信号荧光传感器被开发用于检测有机磷农药（OPs）。它依赖于乙酰胆碱酯酶（AChE）和胆碱氧化酶（ChOx）的催化活性，通过将乙酰胆碱（ACh）转化为胆碱·HO，然后将修饰了二茂铁的四苯乙烯（TPE-Fc）氧化为其氧化态（TPE-Fc），从而产生过氧化氢（HO）。这导致由于聚集而增强的蓝绿色荧光。同时，二茂铁的氧化产生羟基自由基（•OH），导致谷胱甘肽合成的金纳米簇（GSH-AuNCs）的橙色荧光减少。OPs 的存在会限制 AChE 的活性，从而减少 HO 的产生。随着 OPs 浓度的增加，蓝绿色荧光减少，橙色荧光增加，从而实现了对 OPs 的可视化检测。该传感器的线性动态范围为 10-2000ng/mL，检测限为 2.05ng/mL。利用智能手机的颜色识别和微信小程序，成功地进行了快速的 OPs 分析。
