College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
Biosens Bioelectron. 2016 Dec 15;86:971-977. doi: 10.1016/j.bios.2016.07.022. Epub 2016 Jul 12.
Various strategies have been proposed for the sensing of acetylcholinesterase (AChE) activity and organophosphorus pesticides (OPs). However, the practical application of most methods is restricted by their intrinsic drawbacks such as complexity, long analysis time, and high cost. Thus, it is highly desirable to develop simple, fast and sensitive approaches for AChE activity and OPs detection. Herein, we reported a simple paper-based fluorescent sensor (PFS) based on the aggregation induced emission (AIE) effect of tetraphenylethylene (TPE) and the addition reaction capability of maleimide, which has been used as a powerful tool for rapid naked-eye detection of AChE activity and OPs. The introduction of TPE provides the probe with unique fluorescence property in solid state and is of great importance for improving the sensitivity of PFS. The hydrolysis product of acetylthiocholine catalyzed by AChE induced the maleimide ring destruction and activated the fluorescence performance of TPE. Given that AChE activity can be specifically inhibited by OPs, the as-proposed PFS can also be utilized for sensitive detection of OPs. Meanwhile, the variation of fluorescence signal can be readily detected by naked eyes, and low detection limits of 2.5mUmL(-1) and 0.5ngmL(-1) for AChE activity and OPs are obtained, respectively. Moreover, it has been successfully applied for AChE activity and OPs detection in diluted human serum samples, showing its great potential to be applied in real samples. Thus, this strategy possesses considerable advantages of simplicity, rapid detection, portability, cost efficiency and visualization.
已经提出了各种策略来检测乙酰胆碱酯酶（AChE）活性和有机磷农药（OPs）。然而，大多数方法的实际应用受到其内在缺陷的限制，例如复杂性、分析时间长和成本高。因此，开发简单、快速和灵敏的方法来检测 AChE 活性和 OPs 是非常可取的。在此，我们报道了一种基于四苯乙烯（TPE）的聚集诱导发射（AIE）效应和马来酰亚胺加成反应能力的简单纸质荧光传感器（PFS），已将其用作快速肉眼检测 AChE 活性和 OPs 的有力工具。TPE 的引入为探针提供了固态下独特的荧光特性，对提高 PFS 的灵敏度具有重要意义。AChE 催化的乙酰硫代胆碱的水解产物诱导马来酰亚胺环破坏并激活 TPE 的荧光性能。鉴于 AChE 活性可以被 OPs 特异性抑制，所提出的 PFS 也可用于灵敏检测 OPs。同时，荧光信号的变化可以通过肉眼轻松检测到，并且获得了 AChE 活性和 OPs 的低检测限分别为 2.5mUmL(-1)和 0.5ngmL(-1)。此外，它已成功应用于稀释人血清样本中的 AChE 活性和 OPs 检测，显示出其在实际样本中应用的巨大潜力。因此，该策略具有简单、快速检测、便携、经济高效和可视化等优势。
