基于坚固 GeP 纳米片的超灵敏 SPR 生物传感器用于 RNA 检测

An Ultrasensitive SPR biosensor for RNA detection based on robust GeP nanosheets.

机构信息

Center for High Pressure Science, State Key Lab of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.

Institute of Quantum Materials and Devices, School of Electronics and Information Engineering, Tiangong University, Tianjin 300387, China.

出版信息

J Colloid Interface Sci. 2023 Dec;651:938-947. doi: 10.1016/j.jcis.2023.08.064. Epub 2023 Aug 11.

DOI:10.1016/j.jcis.2023.08.064

PMID:37579668

Abstract

Ultrasensitive and rapid detection of biomarkers is among the upmost priorities in promoting healthcare advancements. Improved sensitivity of photonic sensors based on two-dimensional (2D) materials have brought exciting prospects for achieving real-time and label-free biosensing at dilute target concentrations. Here, we report a high-sensitivity surface plasmon resonance (SPR) RNA sensor using metallic 2D GeP nanosheets as the sensing material. Theoretical evaluations revealed that the presence of GeP nanosheets can greatly enhance the plasmonic electric field of the Au film thereby boosting sensing sensitivity, and that optimal sensitivity (146° RIU) can be achieved with 3-nm-thick GeP. By functionalizing GeP nanosheets with specific cDNA probes, detection of SARS-CoV-2 RNA sequences were achieved using the GeP-based SPR sensor, with high sensitivity down to a detection limit of 10 aM and excellent selectivity. This work demonstrates the immense potential of GeP-based SPR sensors for advanced biosensing applications and paves the way for utilizing GeP nanosheets in novel sensor devices.

摘要

超灵敏和快速检测生物标志物是推动医疗保健进步的重中之重。基于二维（2D）材料的光子传感器的灵敏度提高，为实现实时和无标记的生物传感在稀释的目标浓度带来了令人兴奋的前景。在这里，我们报告了一种使用金属 2D GeP 纳米片作为传感材料的高灵敏度表面等离子体共振（SPR）RNA 传感器。理论评估表明，GeP 纳米片的存在可以大大增强 Au 薄膜的等离子体场，从而提高传感灵敏度，并且 3nm 厚的 GeP 可以实现最佳灵敏度（146° RIU）。通过用特异性 cDNA 探针功能化 GeP 纳米片，使用基于 GeP 的 SPR 传感器实现了对 SARS-CoV-2 RNA 序列的检测，检测限低至 10 aM，具有出色的选择性。这项工作展示了基于 GeP 的 SPR 传感器在先进生物传感应用中的巨大潜力，并为在新型传感器设备中利用 GeP 纳米片铺平了道路。