Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 04001, Kosice, Slovakia; College of Photonics, National Yang Ming Chiao Tung University, Tainan 711, Taiwan.
Center for Interdisciplinary Biosciences, Technology, and Innovation Park, P.J. Safarik University in Kosice, Jesenna 5, 041 54, Kosice, Slovakia.
Int J Biol Macromol. 2023 Nov 1;251:126331. doi: 10.1016/j.ijbiomac.2023.126331. Epub 2023 Aug 12.
One of the most attractive approaches in biomedicine and pharmacy is the application of multifunctional materials. The mesoporous structure of clinoptilolite (CZ) absorbs various types of substances and can be used as a model for studying the carriers for targeted drug delivery with controlled release. CZ-dye composites are fabricated by incorporation into clinoptilolite pores commonly used dyes, aluminum phthalocyanine, zinc porphine, and hypericin. We examined and compared the effect of pure dyes and CZ-dye composites on insulin amyloidogenesis. The formation of insulin amyloid fibrils and the disassembly of preformed fibrils is significantly affected by any of the three compounds, however, the strongest effect is observed for aluminum phthalocyanine indicating a structurally-dependent anti-amyloidogenic activity of the dyes. The incorporation of dyes into CZ particles resulted in enhanced anti-amyloidogenic activity in comparison to pure CZ particles. The cell metabolic activity, biocompatibility and fluorescence biodistribution of the dyes entrapped in the composites were tested in vitro (U87 MG cells) and in vivo in the quail chorioallantoic membrane model. Considering the photoactive properties of the dyes used, we assume their applicability in photodiagnostics and photodynamic therapy. It can also be expected that their anti-amyloidogenic potential can be enhanced by photodynamic effect.
在生物医药和药学中，最具吸引力的方法之一是应用多功能材料。斜发沸石（CZ）的中孔结构可吸收各种类型的物质，可作为研究具有控制释放的靶向药物传递载体的模型。通过将常用染料、铝酞菁、锌卟啉和金丝桃素掺入斜发沸石的孔中，制备了 CZ-染料复合材料。我们研究并比较了纯染料和 CZ-染料复合材料对胰岛素淀粉样变性的影响。任何一种化合物都显著影响胰岛素淀粉样原纤维的形成和预形成纤维的解聚，然而，铝酞菁的作用最强，表明染料具有结构依赖性的抗淀粉样变性活性。与纯 CZ 颗粒相比，染料掺入 CZ 颗粒中会增强其抗淀粉样变性活性。体外（U87 MG 细胞）和在鹌鹑尿囊膜模型中体内测试了复合材料中包封的染料的细胞代谢活性、生物相容性和荧光生物分布。考虑到所使用的染料的光活性特性，我们假设它们可用于光诊断和光动力疗法。也可以预期，它们的抗淀粉样变性潜力可以通过光动力效应得到增强。
