Park Sung-Gun, Mohr Jared P, Anderson Gordon A, Bruce James E
Department of Genome Sciences, University of Washington, Seattle, Washington.
GAA Custom Electronics, LLC, Kennewick, Washington.
Rapid Commun Mass Spectrom. 2023 Sep 30;37(18):e9610. doi: 10.1002/rcm.9610.
Hybrid mass spectrometers combine multiple mass analyzers to achieve optimal performance in terms of tandem mass spectrometry, high mass resolving power, and mass measurement accuracy for studying highly complex samples. As a result, the need for transport, trapping, and control of ion kinetic energies is critical for the successful integration of multiple mass analyzers and hybrid instrument operation. In addition, transportation of ion populations between two physically distinct locations can result in time-of-flight (TOF) discrimination against ions with widely disparate m/z values, compromising full mass spectral performance. In this work, we demonstrated a new ion guide, referred to as a planar quadrupole (PQ) ion guide, composed of two parallel printed circuit boards (PCB) that allow radiofrequency (RF) and direct current (DC) voltages to be combined to enable both axial transport and trapping of ion populations in the ultrahigh vacuum region of the mass spectrometer. As compared with a conventional multipole ion guide, the PQ ion guide showed comparable performance in ion m/z values, signal-to-noise, and intensity and effectively reduced mass discrimination caused by TOF effects.
A PQ device was developed with two PCBs and simulated with SIMION 8.1. Electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry instrumentation were used for the testing of PQ performance.
.In this work, we demonstrated a planar quadrupole (PQ) ion guide composed of two parallel PCB plates. The PQ enables both axial ion transport and trapping of ion populations throughout the ion transfer process from a LTQ to an ICR cell. As compared with a conventional multipole ion guide, the PQ showed comparable ion transmission efficiency and effectively reduced mass discrimination caused by TOF effects.
The PQ is a simple design that can be implemented for ion transmission and trapping on virtually any mass spectrometer.
混合质谱仪结合了多个质量分析器，以便在串联质谱分析、高质量分辨能力和质量测量精度方面实现最佳性能，用于研究高度复杂的样品。因此，对于多个质量分析器的成功整合和混合仪器操作而言，离子动能的传输、捕获和控制至关重要。此外，在两个物理上不同的位置之间传输离子群体可能会导致对具有广泛不同m/z值的离子产生飞行时间（TOF）歧视，从而损害全质谱性能。在这项工作中，我们展示了一种新型离子导向器，称为平面四极（PQ）离子导向器，它由两个平行的印刷电路板（PCB）组成，允许将射频（RF）和直流（DC）电压结合起来，以实现离子群体在质谱仪超高真空区域的轴向传输和捕获。与传统的多极离子导向器相比，PQ离子导向器在离子m/z值、信噪比和强度方面表现出相当的性能，并有效减少了由TOF效应引起的质量歧视。
开发了一种带有两个PCB的PQ装置，并用SIMION 8.1进行了模拟。使用电喷雾电离和傅里叶变换离子回旋共振质谱仪器来测试PQ性能。
在这项工作中，我们展示了一种由两个平行PCB板组成的平面四极（PQ）离子导向器。PQ在从线性离子阱（LTQ）到离子回旋共振（ICR）池的整个离子转移过程中，能够实现离子的轴向传输和离子群体的捕获。与传统的多极离子导向器相比，PQ显示出相当的离子传输效率，并有效减少了由TOF效应引起的质量歧视。
PQ设计简单，几乎可以在任何质谱仪上实现离子传输和捕获。
