Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, 200237, Shanghai, China.
Macromol Biosci. 2024 Feb;24(2):e2300282. doi: 10.1002/mabi.202300282. Epub 2023 Sep 10.
Antibacterial hydrogel has emerged as an excellent candidate for wound dressing with the ability to eliminate infection and promote wound healing. Herein, a dynamic hydrogel is developed by Schiff base reaction of mixed charged polypeptides and oxidized dextran (ODex). Specifically, biodegradable polypeptides of 1-(propylthio)acetic acid-3-butylimidazole-modified poly(L-lysine) (PLL-PBIM) and adipate dihydrazide-modified poly(L-glutamic acid) (PLG-ADH) are achieved with tunable substitution and charge. By mixing with ODex, charged polypeptides of PLL-PBIM and PLG-ADH led to an injectable and self-healing hydrogel in seconds. The injectable and self-healing performances of the hydrogels are ascribed to the reversible imine and hydrazone bonds formed between polypeptides and ODex. The positively charged hydrogels exhibited over 95% antibacterial activity against E. coli and S. aureus. An optimized balancing of PLG-ADH and PLL-PBIM significantly reduced the hemolysis rate and cytotoxicity of hydrogels. Therefore, the dynamic hydrogel with excellent biocompatibility and inherently antibacterial ability can have potential application for wound dressing.
抗菌水凝胶作为一种理想的伤口敷料，具有消除感染和促进伤口愈合的能力。本文通过混合带电荷多肽与氧化葡聚糖（ODex）的席夫碱反应，制备了一种动态水凝胶。具体而言，通过 1-（丙硫基）乙酸-3-丁基咪唑修饰聚 L-赖氨酸（PLL-PBIM）和己二酸二酰肼修饰聚 L-谷氨酸（PLG-ADH）的生物可降解多肽，实现了可调取代和电荷。与 ODex 混合后，带电荷的 PLL-PBIM 和 PLG-ADH 多肽在几秒钟内形成了可注射和自修复水凝胶。水凝胶的可注射和自修复性能归因于多肽和 ODex 之间形成的可逆亚胺键和腙键。带正电荷的水凝胶对大肠杆菌和金黄色葡萄球菌的抑菌率超过 95%。PLG-ADH 和 PLL-PBIM 的优化平衡显著降低了水凝胶的溶血率和细胞毒性。因此，具有优异生物相容性和固有抗菌能力的动态水凝胶可在伤口敷料中有潜在应用。
