Xue-Li Song -, Guo Feng -, Lai-Lai Li -, Wei Li -, Zeng-Guang Wu -, Wen-Jing Wang -, Hong-Mei Su -, Chen-Chen Ren -, Guang-Lin Zhu -, Zheng-Yan He -, Ju Zhang -
Department of Chinese Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, P.R. China.
Pak J Pharm Sci. 2023 May;36(3):801-809.
Based on metabolomics, to study the mechanism of Radix Wikstroemia indica (RWI) "Sweat soaking method" processing detoxification. The raw drug group and processed products was given raw RWI and processed RWI respectively by gavage. The control group was given the same amount of 1% sodium carboxy methyl cellulose distilled water by gavage. After 7 days of continuous gavage, blood samples were collected. The blood samples of rats in each group were analyzed by H-NMR technology to explore the changes of endogenous metabolism and the possible metabolic pathways to rats before and after processing. Compared with the control group, the raw RWI could significantly reduce 16 metabolites and increase 10 metabolites. The processed RWI can increase the levels of most metabolites that decrease to the raw RWI, such as 13 metabolites such as alanine, L-glutamine, L-valine, L-serine, betaine and glutamic acid; At the same time, the metabolites that increased in the level of crude products were down-regulated, such as asparagine, lactic acid, 2-hydroxyisobutyric acid, sucrose, glucose and D-glucose. Compared with raw products, RWI treated with "Sweat soaking method" can reversely regulate or reduce amino acid, choline metabolism, energy and carbohydrate metabolism, thereby reducing hepatotoxicity and nephrotoxicity.
基于代谢组学，研究了黄芫花“汗浸法”炮制减毒的机制。分别将生黄芫花和炮制品灌胃给予生药组和炮制品组。对照组给予等量的1%羧甲基纤维素钠蒸馏水灌胃。连续灌胃7天后，采集血样。采用氢核磁共振（H-NMR）技术分析各组大鼠血样，探讨炮制前后大鼠内源性代谢变化及可能的代谢途径。与对照组相比，生黄芫花可显著降低16种代谢物含量，增加10种代谢物含量。炮制后的黄芫花可使生黄芫花降低的多数代谢物含量升高，如丙氨酸、L-谷氨酰胺、L-缬氨酸、L-丝氨酸、甜菜碱和谷氨酸等13种代谢物；同时，使生品中含量升高的代谢物下调，如天冬酰胺、乳酸、2-羟基异丁酸、蔗糖、葡萄糖和D-葡萄糖。与生品相比，“汗浸法”炮制的黄芫花可反向调节或降低氨基酸、胆碱代谢、能量和碳水化合物代谢，从而降低肝毒性和肾毒性。
