College of Pharmacy, Yanbian University, Yanji, China.
College of Pharmacy, Yanbian University, Yanji, China/School of Pharmacy, Baicheng Medical College, Baicheng, China.
Pak J Pharm Sci. 2023 May;36(3):819-827.
Gastric ulcer is a common gastrointestinal disease caused by excessive gastric acid secretion, which has been recognized as one of the most common causes of morbidity and mortality in the world. The skin of Rana chensinensis is rich in collagen and many previous studies have shown that it has certain bioactivity. Therefore, we extracted and purified collagen with a molecular weight less than 10000 Da from the skin of Rana chensinensis, and studied its gastric protective mechanism through the model of ethanol-induced gastric ulcer in Balb/c mice. The results showed that through macroscopic observation and significantly reduced ulcer index, it was proved that PCRCS could protect gastric mucosa and alleviate the damage of ethanol to gastric mucosa. PCRCS reduced ethanol-induced oxidative stress by boosting depleted SOD levels and dramatically lowering MDA levels, as well as significantly reducing lipid peroxidation. Additionally PCRCS (Protein Chinese Rana chesinensis Skin) additionally decreased the launch of inflammatory mediators TNF-α and IL-6 and more desirable the content material of protective elements NO and PGE2 in gastric mucosa. Based on these findings, we believe that PCRCS has potential stomach protective effects on ethanol-induced gastric ulcer, which may be achieved by inhibiting oxidative stress and stomach inflammation.
胃溃疡是一种常见的胃肠道疾病，是由胃酸分泌过多引起的，已被认为是世界上发病率和死亡率最高的疾病之一。中华大蟾蜍的皮肤富含胶原蛋白，许多先前的研究表明其具有一定的生物活性。因此，我们从中华大蟾蜍的皮肤中提取并纯化了分子量小于 10000Da 的胶原蛋白，并通过乙醇诱导的 Balb/c 小鼠胃溃疡模型研究了其胃保护机制。结果表明，通过宏观观察和显著降低溃疡指数，证明 PCRCS 可以保护胃黏膜，减轻乙醇对胃黏膜的损伤。PCRCS 通过提高耗竭的 SOD 水平和显著降低 MDA 水平以及显著减少脂质过氧化来减轻乙醇诱导的氧化应激。此外，PCRCS（中华大蟾蜍皮肤蛋白）还减少了促炎介质 TNF-α 和 IL-6 的释放，并使胃黏膜中保护因子 NO 和 PGE2 的含量更加理想。基于这些发现，我们认为 PCRCS 对乙醇诱导的胃溃疡具有潜在的胃保护作用，其可能通过抑制氧化应激和胃炎症来实现。
