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通过微阵列分析过氧化氢处理的胰岛衍生 1.1B4 细胞和靶向 NR4A3 的 siRNA 处理的细胞的抗氧化防御系统。

Analysis of an antioxidative defence system of hydrogen peroxide-treated pancreatic islet-derived 1.1B4 cells and siRNA targeting NR4A3-treated cells by microarray.

机构信息

Department of Life Science, Graduate School, Ochanomizu University, Tokyo, Japan.

School of Health Science, Faculty of Medicine, Tottori University, Tottori, Japan.

出版信息

Redox Rep. 2023 Dec;28(1):2247150. doi: 10.1080/13510002.2023.2247150.

DOI:10.1080/13510002.2023.2247150
PMID:37581334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10435006/
Abstract

Pancreatic islet β-cells weaken under oxidative stress. In this study, human pancreatic islet-derived 1.1B4 cells were exposed to HO and analysed using a human microarray, which revealed that (, , , () and were upregulated, whereas and were not. Expression of rapidly increased after HO addition, and the 1.1B4 cells treated with siRNA targeting became sensitive to HO; further, expression was strongly inhibited, suggesting that is an oxidative stress-responsive transcription factor that functions through expression in pancreatic islet β-cells. Expression of and was also inhibited by siRNAs targeting .

摘要

胰岛β细胞在氧化应激下功能减弱。在这项研究中，我们将人胰岛衍生的 1.1B4 细胞暴露于 H2O2 中，并使用人类微阵列进行分析，结果显示， 、 、 、 （ ）和 上调，而 和 没有上调。H2O2 处理后， 表达迅速增加，用针对 的 siRNA 处理的 1.1B4 细胞对 H2O2 敏感；此外， 表达被强烈抑制，表明 是一种氧化应激反应性转录因子，通过在胰岛β细胞中表达 发挥作用。针对 的 siRNAs 也抑制 和 的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/c834e2753b95/YRER_A_2247150_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/c9b1efbd6642/YRER_A_2247150_UF0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/c5e2d796ef9a/YRER_A_2247150_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/dfafec7c9587/YRER_A_2247150_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/8ffd05b315dd/YRER_A_2247150_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/c834e2753b95/YRER_A_2247150_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/c9b1efbd6642/YRER_A_2247150_UF0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/c5e2d796ef9a/YRER_A_2247150_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/dfafec7c9587/YRER_A_2247150_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/8ffd05b315dd/YRER_A_2247150_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6965/10435006/c834e2753b95/YRER_A_2247150_F0004_OC.jpg

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本文引用的文献

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