Mazière C, Mazière J C, Mora L, Polonovski J
Exp Cell Res. 1986 Nov;167(1):257-61. doi: 10.1016/0014-4827(86)90222-3.
Phosphatidyl choline (PC) synthesis in trypsinized and reattaching fibroblasts during the spreading state was studied by incorporation of [14C]choline and [methyl-14C]methionine. The choline and phosphatidyl-ethanolamine (PE) transmethylation pathways were both transiently increased about 2-fold during the first 2 h after replating. Maximum increase appeared to be simultaneous with maximum spreading. Incorporation of [32P]orthophosphate showed that the increase in PC synthesis was specific and most probably related to establishment of cell-substrate adhesion sites.
通过掺入[14C]胆碱和[甲基-14C]甲硫氨酸，研究了胰蛋白酶消化后重新附着的成纤维细胞在铺展状态下磷脂酰胆碱（PC）的合成。在重新接种后的最初2小时内，胆碱和磷脂酰乙醇胺（PE）的甲基转移途径均短暂增加了约2倍。最大增加似乎与最大铺展同时出现。掺入[32P]正磷酸盐表明，PC合成的增加是特异性的，很可能与细胞-底物粘附位点的建立有关。
