Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Department of Chemistry, Moscow State University, Moscow, Russia.
Dokl Biochem Biophys. 2023 Jun;510(1):104-109. doi: 10.1134/S1607672923700205. Epub 2023 Aug 15.
Telomerase is a ribonucleoprotein complex, the main components of which are telomerase RNA and reverse transcriptase. Previously, it was shown in our laboratory that human telomerase RNA contains an open reading frame starting at adenine in position 176. The open reading frame encodes the hTERP protein, and the deletion of nucleotides 184-188 of human telomerase RNA disrupts the open reading frame and leads to the absence of hTERP. Human telomerase RNA has a conserved structure, changes in which affect telomerase activity. In this work, we have shown that the deletion of nucleotides 184-188 of telomerase RNA does not affect the functioning of telomerase.
端粒酶是一种核糖核蛋白复合物，其主要成分是端粒酶 RNA 和逆转录酶。此前，我们实验室的研究表明，人类端粒酶 RNA 包含一个从 176 位腺嘌呤开始的开放阅读框。该开放阅读框编码 hTERP 蛋白，而人类端粒酶 RNA 中 184-188 个核苷酸的缺失会破坏开放阅读框，导致 hTERP 蛋白的缺失。人类端粒酶 RNA 具有保守的结构，其结构的变化会影响端粒酶的活性。在这项工作中，我们已经证明端粒酶 RNA 中 184-188 个核苷酸的缺失不会影响端粒酶的功能。
