Musset Olivier, Balourdet Aude, Perrot-Minnot Marie-Jeanne
Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078, Dijon, France.
Biogéosciences, UMR 6282 CNRS, Université de Bourgogne, 6 Boulevard Gabriel, 21000, Dijon, France.
Curr Res Parasitol Vector Borne Dis. 2023 Jul 26;4:100135. doi: 10.1016/j.crpvbd.2023.100135. eCollection 2023.
Clearing infection is an essential step to address many issues in host-parasite interactions but is challenging when dealing with endoparasites of large size relative to that of their host. Here, we took advantage of the lethality, contactless and versatility of high-energy laser beam to achieve it, using thorny-headed worms (Acanthocephala) and their amphipod intermediate host as a model system. We show that laser-based de-parasitization can be achieved using 450 nm Blue Diode Laser targeting carotenoid pigments in the bird acanthocephalan . Using proboscis evagination failure and DNA degradation to establish parasite death, we found that 80% died from within-host exposure to 5 pulses of 50 ms duration, 1.4 W power. Survival of infected gammarids 11 days after laser treatment was 60%. Preliminary tests were also performed with Nanosecond-Green Laser targeting lipids in another acanthocephalan parasite. We discuss the efficiency and side-effect of laser treatment in this host-parasite system and highlight the perspectives that this technology more generally offers in parasitology.
清除感染是解决宿主-寄生虫相互作用中许多问题的关键步骤，但在处理相对于宿主而言体型较大的体内寄生虫时具有挑战性。在此，我们利用高能激光束的致死性、非接触性和多功能性来实现这一目标，以棘头虫（棘头动物门）及其双足类中间宿主作为模型系统。我们表明，使用450纳米蓝色二极管激光靶向鸟类棘头虫中的类胡萝卜素色素，可以实现基于激光的去寄生虫操作。利用吻突外翻失败和DNA降解来确定寄生虫死亡，我们发现80%的寄生虫在宿主体内暴露于5个持续时间为50毫秒、功率为1.4瓦的脉冲后死亡。激光治疗11天后，受感染的双足类动物的存活率为60%。我们还使用纳秒级绿色激光靶向另一种棘头虫寄生虫中的脂质进行了初步测试。我们讨论了激光治疗在这个宿主-寄生虫系统中的效率和副作用，并强调了这项技术在寄生虫学中更广泛提供的前景。
