Marchesini S, Benaglia G, Piccinotti A, Bresciani R, Preti A
Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
FEBS Lett. 1998 May 22;428(1-2):115-7. doi: 10.1016/s0014-5793(98)00510-9.
GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5-24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence of fluorescent GM3 is consistent with the presence in these cells of a sialidase working on GM1 and GM2 gangliosides. After treatment of the cells with chloroquine the fluorescent GM1 remained essentially undegraded, indicating a catabolic processing at lysosomal level.
携带荧光脂肪酸以替代天然脂肪酸的GM1神经节苷脂，已在不同脉冲时间（0.5 - 24小时）施用于培养的马-达二氏犬肾（MDCK）细胞，并追踪其代谢命运。荧光GM2、脱唾液酸GM2、脱唾液酸GM1和神经酰胺是仅可检测到的代谢产物。荧光GM3的完全缺失与这些细胞中存在作用于GM1和GM2神经节苷脂的唾液酸酶一致。用氯喹处理细胞后，荧光GM1基本未降解，表明在溶酶体水平进行分解代谢过程。
