He Hairong, Huang Jiarui, Zhao Zhenzhu, Feng Weisheng, Zheng Xiaoke, Du Pengqiang
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China.
College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
Front Microbiol. 2023 Jul 31;14:1198808. doi: 10.3389/fmicb.2023.1198808. eCollection 2023.
Bacterial communities are important for soil functions, but the effect of clomazone on network complexity, composition, and stability is not well studied.
In this study, two agricultural soils were used to test the impact of clomazone on bacterial communities, and the two soils were treated with three concentrations of clomazone (0, 0.8, 8, and 80 mg kg) in an incubator.
Bacterial network nodes, links, and average degrees were all decreased by 9-384, 648-829, and 0.703-2.429, respectively. Based on keystone nodes, the topological roles of the nodes were also influenced by clomazone. Bacterial network composition was also impacted based on the analysis of similarity (ANOSIM) and network dissimilarity. Compared with control and clomazone treatments in both soils, the ANOSIM between control and all clomazone treatments was higher than 0.6, network dissimilarities were 0.97-0.98, shared nodes were 131-260, and shared links were 12-100. The bacterial network stability was decreased by clomazone, with decreased robustness by 0.01-0.016 and increased vulnerability by 0.00023-0.00147 in both soils. There were fewer bacterial network modules preserved after clomazone treatment, and the bacterial network community functions were also impacted in both soils. Based on these results, soil bacterial species connections, modularization, and network stability were significantly impacted by clomazone.
细菌群落对土壤功能很重要，但广灭灵对网络复杂性、组成和稳定性的影响尚未得到充分研究。
在本研究中，使用两种农业土壤来测试广灭灵对细菌群落的影响，将这两种土壤在培养箱中用三种浓度的广灭灵（0、0.8、8和80毫克/千克）进行处理。
细菌网络的节点、连接和平均度分别降低了9 - 384、648 - 829和0.703 - 2.429。基于关键节点，节点的拓扑作用也受到广灭灵的影响。基于相似性分析（ANOSIM）和网络差异分析，细菌网络组成也受到了影响。与两种土壤中的对照和广灭灵处理相比，对照与所有广灭灵处理之间的ANOSIM高于0.6，网络差异为0.97 - 0.98，共享节点为131 - 260，共享连接为12 - 100。广灭灵降低了细菌网络的稳定性，两种土壤中的稳健性降低了0.01 - 0.016，脆弱性增加了0.00023 - 0.00147。广灭灵处理后保留的细菌网络模块减少，两种土壤中的细菌网络群落功能也受到了影响。基于这些结果，广灭灵对土壤细菌物种的连接、模块化和网络稳定性有显著影响。
