Wang Zihao, Guo Jiale, Fan Qi, Tian Zeyuan, Wang Xueqing, Zheng Wei, Huang Luodong
College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Jul 25;39(7):2926-2938. doi: 10.13345/j.cjb.220890.
is a widely cultivated horticultural plant in the world, which has high ornamental and medicinal value. In this study, the whole genome of leaves from . was sequenced by Illumina HiSeq 4000 platform. The chloroplast genome were assembled for functional annotation, sequence characteristics and phylogenetic analysis. The results showed that the chloroplast genome of . composed of four regions with a size of 154 926 bp, which was the smallest chloroplast genome reported for species to date. A total of 132 genes were identified, including 86 coding genes, 38 tRNA genes and 8 rRNA genes. Codon bias analysis found that the codon usage bias was weak and there was a bias for using A/U base endings. 46 simple sequence repeat and 54 repeats loci were detected in the chloroplast genome, with the maximum detection rate in the large single copy region and inverted repeat region, respectively. The inverted repeats boundaries of . and were highly conserved, whereas gene location differences occurred. Phylogenetic analysis revealed that . and . form a monophyletic clade, which was the closest relationship and conformed to the morphological classification characteristics. The analysis of the chloroplast genome of . provides important data basis for species identification, genetic diversity and chloroplast genome engineering of .
是世界上广泛种植的园艺植物，具有很高的观赏和药用价值。在本研究中，通过Illumina HiSeq 4000平台对[植物名称]叶片的全基因组进行了测序。对叶绿体基因组进行组装，用于功能注释、序列特征和系统发育分析。结果表明，[植物名称]的叶绿体基因组由四个区域组成，大小为154926 bp，是迄今为止报道的该物种最小的叶绿体基因组。共鉴定出132个基因，包括86个编码基因、38个tRNA基因和8个rRNA基因。密码子偏好性分析发现密码子使用偏好较弱，且存在使用A/U碱基结尾的偏好。在叶绿体基因组中检测到46个简单序列重复和54个重复序列位点，分别在大单拷贝区域和反向重复区域检测率最高。[植物名称]和[另一植物名称]的反向重复边界高度保守，但基因位置存在差异。系统发育分析表明，[植物名称]和[另一植物名称]形成一个单系分支，关系最为密切，符合形态学分类特征。对[植物名称]叶绿体基因组的分析为该物种的鉴定、遗传多样性和叶绿体基因组工程提供了重要的数据基础。
