Turdi Rayhangul, Mu Lihong, Tian Xinmin
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Aug 25;38(8):2999-3013. doi: 10.13345/j.cjb.210876.
In order to characterize the chloroplast genome and phylogenetic relationships of , we performed Illumina Hiseq high-throughput sequencing to sequence the complete chloroplast genome of this plant and constructed a whole-genome map based on contig assembly and annotation. The chloroplast genome of . is 161 034 bp in length and has a typical tetrad structure, comprising 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The genome also contains a total of 44 dispersed repeat sequences and 47 simple sequence repeats. Among the genome's 53 678 codons, the largest proportion are leucine-encoding codons (5 251), whereas the smallest proportion encode tryptophan (712). Colinear analysis revealed an absence of inversions and rearrangements between . and related species at the chloroplast genome level. Whereas phylogenetic analysis indicated that . did not cluster in a clade with , it did show a very close phylogenetic relationship with . The findings of this study provide basic data that will contribute to further species identification and phylogenetic study of the genus .
为了表征[植物名称]的叶绿体基因组及其系统发育关系,我们进行了Illumina Hiseq高通量测序,以对该植物的完整叶绿体基因组进行测序,并基于重叠群组装和注释构建了全基因组图谱。[植物名称]的叶绿体基因组长度为161034 bp,具有典型的四分体结构,包括85个蛋白质编码基因、37个tRNA基因和8个rRNA基因。该基因组还总共包含44个分散重复序列和47个简单序列重复。在基因组的53678个密码子中,比例最大的是编码亮氨酸的密码子(5251个),而比例最小的是编码色氨酸的密码子(712个)。共线性分析表明,在叶绿体基因组水平上,[植物名称]与相关物种之间不存在倒位和重排。而系统发育分析表明,[植物名称]没有与[另一植物名称]聚类在一个进化枝中,但它与[另一植物名称]显示出非常密切的系统发育关系。本研究结果提供了基础数据,将有助于该属植物的进一步物种鉴定和系统发育研究。