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Preparation and purification of the proteinase inhibitor, leupeptin, from culture filtrates of Streptomyces lavendulae.

作者信息

Ning M C, Beynon R J

出版信息

Int J Biochem. 1986;18(9):813-20. doi: 10.1016/0020-711x(86)90058-3.

DOI:10.1016/0020-711x(86)90058-3
PMID:3758463
Abstract

Leupeptin (N-acetyl-L-leucyl-L-leucyl-L-argininal) was produced as an extracellular metabolite by Streptomyces lavendulae. Addition of lysine to the growth medium stimulated leupeptin production from 200 to 1400 micrograms/ml. Incubation of late exponential phase mycelium in a synthetic medium was used to prepare single labelled (3H) and double labelled (3H/14C) inhibitor for metabolic studies. An improved purification scheme that generates leupeptin of high purity with good recoveries is also reported.

摘要

相似文献

1
Preparation and purification of the proteinase inhibitor, leupeptin, from culture filtrates of Streptomyces lavendulae.
Int J Biochem. 1986;18(9):813-20. doi: 10.1016/0020-711x(86)90058-3.
2
Kinetic study on the production and degradation of leupeptin in Streptomyces exfoliatus SMF13.
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3
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The inhibition of the enzymic activity of blood coagulation and fibrinolytic serine proteases by a new leupeptin-like inhibitor, and its structural analogues, isolated from Streptomyces griseus.
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Physiological roles of leupeptin and extracellular proteases in mycelium development of Streptomyces exfoliatus SMF13.亮抑酶肽和细胞外蛋白酶在脱落链霉菌SMF13菌丝体发育中的生理作用
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6
[Action of proteinase inhibitors in rats. 3. Influence of leupeptin on the rate of protein synthesis and the intracellular protein degradation by use of a test system including constant infusion of labelled amino acids, estimation of 3-methyl-histidine excretion and a triple-labelling technique].[蛋白酶抑制剂在大鼠体内的作用。3. 通过使用包括持续输注标记氨基酸、测定3-甲基组氨酸排泄量以及三重标记技术的测试系统，研究亮抑酶肽对蛋白质合成速率和细胞内蛋白质降解的影响]
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Biosynthesis of leupeptin. IV. Is protein turnover in leupeptin producer cells affected by leupeptin?
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9
Trypsin-like protease of Streptomyces exfoliatus SMF13, a potential agent in mycelial differentiation.
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J Bacteriol. 1998 Mar;180(5):1334-7. doi: 10.1128/JB.180.5.1334-1337.1998.

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