McDonald S A
Dev Biol. 1986 Oct;117(2):546-9. doi: 10.1016/0012-1606(86)90322-2.
The center-initiating behavior of Dictyostelium discoideum amoebae in various cell-cycle phases was investigated. Small populations of synchronized AX-2 cells were seeded 1 in 1000 into cultures of a nonsignaling mutant (NP160) incapable of initiating centers. The ability of the wild-type AX-2 cells to initiate centers among mutant amoebae displayed cell-cycle regulation. Approximately 50% of a population of S-phase cells initiated centers while only 7.5% of a population of late G2-phase cells resulted in center formation. The timing of center formation also varied with cycle position. Synchronous cultures containing only AX-2 S-phase amoebae (no NP160) displayed the initial signs of aggregation after 4.5 hr of starvation and streaming into the aggregate was complete after 6 hr. In contrast, cultures of late G2-phase amoebae initiated aggregation centers after 5.5 hr of starvation and did not complete streaming until 7.5 hr. In addition, the number of aggregates formed by these synchronous cultures of AX-2 cells also varied with cycle position. In general, these results suggest a cell-cycle modulation of the autonomous signaling responsible for center initiation.
研究了盘基网柄菌变形虫在不同细胞周期阶段的中心启动行为。将少量同步化的AX - 2细胞以1:1000的比例接种到不能启动中心的无信号突变体（NP160）的培养物中。野生型AX - 2细胞在突变体变形虫中启动中心的能力表现出细胞周期调控。大约50%的S期细胞群体启动了中心，而只有7.5%的晚G2期细胞群体形成了中心。中心形成的时间也随细胞周期位置而变化。仅含有AX - 2 S期变形虫（无NP160）的同步培养物在饥饿4.5小时后显示出聚集的初始迹象，并且在6小时后流入聚集体的过程完成。相比之下，晚G2期变形虫的培养物在饥饿5.5小时后启动聚集中心，直到7.5小时才完成流动。此外，这些AX - 2细胞的同步培养物形成的聚集体数量也随细胞周期位置而变化。总体而言，这些结果表明负责中心启动的自主信号存在细胞周期调节。
