Convatec Limited, Deeside, Flintshire, United Kingdom.
Surg Infect (Larchmt). 2023 Sep;24(7):637-644. doi: 10.1089/sur.2023.155. Epub 2023 Aug 16.
Surgical site infections can lead to serious complications and present a huge economic burden. Established wound infections can be difficult to eradicate so preventative measures, including antimicrobial dressings, are advantageous. The antimicrobial activity of an ionic silver, ethylenediaminetetraacetic acid (EDTA) and benzethonium chloride-containing (ISEB) surgical cover dressing (SCD) was compared with two other silver-containing SCDs (silver sulfate and ionic silver carboxymethylcellulose [CMC]) and a non-silver-containing CMC SCD control using an in vitro model. The dressings were tested against a range of gram-positive and gram-negative bacteria found in wound environments, including antibiotic resistant strains, using a direct inoculation simulated wound fluid (SWF) model. Dressings were fully hydrated with SWF and inoculated with a final concentration of 1 × 10 colony forming units (CFU) per 10 microliter of the challenge organisms. Dressings were incubated at 35°C ± 3°C for up to seven days; total viable counts (TVCs) were performed to determine bacterial bioburden. All challenge organism levels remained high for the CMC SCD control and silver sulfate SCD throughout the test period. A greater than 95% reduction in TVCs was observed by four hours for all challenge organisms for the ISEB SCD, with non-detectable levels (<70 CFU per dressing) reached within 24 hours and sustained throughout the test period. Antimicrobial activity was less rapid with ionic silver CMC SCD, with 9 of 11 challenge organisms reaching undetectable levels within 6 to 72 hours. A more rapid antimicrobial activity was observed for the ISEB SCD compared with other dressings tested within this in vitro model.
手术部位感染可导致严重并发症，并带来巨大的经济负担。已确立的伤口感染很难消除，因此预防措施，包括使用抗菌敷料，是有利的。 本研究比较了一种含离子银、乙二胺四乙酸（EDTA）和苯扎氯铵（ISEB）的外科覆盖敷料（SCD）与两种其他含银的 SCD（硫酸银和离子银羧甲基纤维素[CMC]）以及一种不含银的 CMC SCD 对照在体外模型中，用模拟伤口液（SWF）模型对敷料进行了测试，测试范围包括伤口环境中发现的各种革兰氏阳性和革兰氏阴性细菌，包括抗生素耐药菌株。将敷料用 SWF 完全水化，并以每 10 微升挑战生物的最终浓度 1×10 个菌落形成单位（CFU）接种。将敷料在 35°C±3°C下孵育长达七天；进行总活菌计数（TVC）以确定细菌生物负荷。 在整个测试期间，CMC SCD 对照和硫酸银 SCD 的所有挑战生物水平都保持较高水平。ISEB SCD 在所有挑战生物的 4 小时内观察到 TVC 减少了 95%以上，在 24 小时内达到不可检测水平（<70 CFU/敷料），并在整个测试期间持续。离子银 CMC SCD 的抗菌活性较慢，11 个挑战生物中的 9 个在 6 至 72 小时内达到不可检测水平。 在该体外模型中，与其他测试的敷料相比，ISEB SCD 观察到更快的抗菌活性。
