Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Neurosci Lett. 2023 Sep 14;813:137436. doi: 10.1016/j.neulet.2023.137436. Epub 2023 Aug 14.
This study aimed to determine effects of the resveratrol on ketamine-induced schizophrenia-like behaviors and oxidative damage in mice. Twenty-four male mice were allocated into four experimental groups as control, ketamine (20 mg/kg), resveratrol (80 mg/kg) and co-administration of the ketamine (20 mg/kg) + resveratrol (80 mg/kg). Mice were received resveratrol for 30 days and ketamine was used for an animal model of schizophrenia and was injected from days 16 to 30 of the study. After the drug administration was finished, schizophrenia-like behaviors were evaluated using object recognition test, tail suspension test, forced swimming test and open field test and brain malondialdehyde, glutathione peroxidase, superoxide dismutase and catalase levels were determined. According to the results, ketamine treatment significantly decreased body weight and pretreatment with resveratrol elevated body weight compared to ketamine group (P < 0.05). Ketamine treatment significantly decreased number of the cross in open field test and pretreatment with resveratrol improved i (P < 0.05). Immobility time in tail suspension and forced swimming tests increased in mice treated with ketamine (P < 0.05). Pretreatment with resveratrol diminished immobility time compared to ketamine group (P < 0.05). Ketamine significantly decreased memory deficits while pretreatment with resveratrol significantly reduced the memory deficits induced by ketamine (P < 0.05). Brain MDA increased in both cortical and sub-cortical area in ketamine treated mice while pretreatment with resveratrol decreased ketamine-induced elevation in MDA (P < 0.05). Ketamine significantly decreased brain SOD, GPx and CAT levels while pretreatment with resveratrol improved SOD, GPx and CAT levels (P < 0.05). Findings suggested resveratrol has neuroprotective effects against ketamine-induced behavioral deficits and oxidative damages.
这项研究旨在确定白藜芦醇对氯胺酮诱导的似精神分裂症行为和氧化损伤的影响。将 24 只雄性小鼠分配到对照组、氯胺酮（20mg/kg）、白藜芦醇（80mg/kg）和氯胺酮（20mg/kg）+白藜芦醇（80mg/kg）联合给药组 4 个实验组中。小鼠接受白藜芦醇治疗 30 天，氯胺酮用于研究第 16 至 30 天的精神分裂症动物模型，并进行注射。给药结束后，使用物体识别试验、悬尾试验、强迫游泳试验和旷场试验评估似精神分裂症行为，并测定脑丙二醛、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶水平。结果表明，与氯胺酮组相比，氯胺酮治疗显著降低了体重，而白藜芦醇预处理增加了体重（P<0.05）。氯胺酮治疗显著减少了旷场试验中的十字交叉次数，而白藜芦醇预处理改善了这一指标（P<0.05）。氯胺酮治疗组小鼠的悬尾和强迫游泳试验中不动时间增加（P<0.05），而白藜芦醇预处理组的不动时间较氯胺酮组减少（P<0.05）。氯胺酮显著降低了记忆缺损，而白藜芦醇预处理显著降低了氯胺酮引起的记忆缺损（P<0.05）。氯胺酮治疗导致皮质和皮质下区域脑 MDA 增加，而白藜芦醇预处理降低了氯胺酮诱导的 MDA 升高（P<0.05）。氯胺酮显著降低了脑 SOD、GPx 和 CAT 水平，而白藜芦醇预处理改善了 SOD、GPx 和 CAT 水平（P<0.05）。研究结果表明，白藜芦醇对氯胺酮诱导的行为缺陷和氧化损伤具有神经保护作用。
