Department of Chemical and Materials Engineering, Chang Gung University, Guishan, Taoyuan 33302, Taiwan; Division of Nephrology, Department of Internal Medicine, Chang Gung Memorial Hospital Linkou, Taoyuan 33305, Taiwan; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chungli, Taoyuan 32003, Taiwan.
Department of Chemical and Materials Engineering, Chang Gung University, Guishan, Taoyuan 33302, Taiwan; Division of Nephrology, Department of Internal Medicine, Chang Gung Memorial Hospital Linkou, Taoyuan 33305, Taiwan; Department of Safety, Health and Environmental Engineering, Ming Chi University of Technology, Taishan, New Taipei City 24301, Taiwan.
Int J Biol Macromol. 2023 Nov 1;251:126337. doi: 10.1016/j.ijbiomac.2023.126337. Epub 2023 Aug 15.
Thermo-/pH-sensitive nanocomposites based on mesoporous silicate MCM-41 (MSNCs) derived from rice husk ash were synthesized and characterized. MSNCs were coated with thermo-/pH-sensitive Pluronic® F127 and gelatin to form MSNCs@gp nanocomposites, serving as carriers for controlled release of the anticancer drug doxorubicin (Dox). The in vitro and in vivo antitumor efficacy of MSNCs@gp-Dox against liver cancer was evaluated. Fourier-transform infrared (FTIR) spectra confirmed the silica nature of MSNCs@gp by detecting the Si-O-Si group. Under acidic microenvironments (pH 5.4) and 42 °C, MSNCs@gp-Dox exhibited significantly higher Dox release (47.33 %) compared to physiological conditions. Thermo-/pH-sensitive drug release (47.33 %) was observed in simulated tumor environments. The Makoid-Banakar model provided the best fit at pH 7.4 and 37 °C with a mean squared error of 0.4352, an Akaike Information Criterion of 15.00, and a regression coefficient of 0.9972. Cytotoxicity tests have demonstrated no significant toxicity in HepG2 cells treated with various concentrations of MSNCs@gp, while MSNCs@gp-Dox induced considerable cell apoptosis. In vivo studies in nude mice revealed effective suppression of liver cancer growth by MSNCs@gp-Dox, indicating high pharmaceutical efficacy. The investigated MSNCs@gp-based drug delivery system shows promise for liver cancer therapy, offering enhanced treatment efficiency with minimal side effects.
以稻壳灰衍生的介孔硅 MCM-41（MSNCs）为基础，合成并表征了温敏/酸敏纳米复合材料。MSNCs 被温敏/酸敏的 Pluronic® F127 和明胶包覆，形成 MSNCs@gp 纳米复合材料，作为载体制备用于控制阿霉素（Dox）的抗癌药物释放。评估了 MSNCs@gp-Dox 对肝癌的体外和体内抗肿瘤功效。傅里叶变换红外（FTIR）光谱通过检测 Si-O-Si 基团证实了 MSNCs@gp 的硅性质。在酸性微环境（pH 5.4）和 42°C 下，MSNCs@gp-Dox 表现出明显更高的阿霉素释放（47.33%），与生理条件相比。在模拟肿瘤环境中观察到温敏/酸敏药物释放（47.33%）。Makoid-Banakar 模型在 pH 7.4 和 37°C 下提供了最佳拟合，均方误差为 0.4352，Akaike 信息准则为 15.00，回归系数为 0.9972。细胞毒性试验表明，用不同浓度的 MSNCs@gp 处理 HepG2 细胞没有明显毒性，而 MSNCs@gp-Dox 诱导了相当大的细胞凋亡。裸鼠体内研究表明，MSNCs@gp-Dox 能有效抑制肝癌的生长，表明具有较高的药物疗效。所研究的基于 MSNCs@gp 的药物传递系统显示出用于肝癌治疗的潜力，提供了增强的治疗效率和最小的副作用。
