Department of Physiology, Delta State University, Abraka, Delta State, Nigeria.
Department of Nursing, Delta State University, Abraka, Delta State, Nigeria.
Sci Rep. 2023 Aug 16;13(1):13309. doi: 10.1038/s41598-023-40454-5.
The cellular integrity of the kidney in homeostatic regulation has constantly been compromised by oxidative stress following exposure to varying nature of stressor present within the environment. The objective of the work was to evaluate the renal effect of the different stressor stimuli applied. Twenty-four adult female rats weighing averagely 160-200 g and within the ages of 12-14 weeks were used for experiment-1, while 12 offspring were utilized for experiment-2. Three stress models namely; restraint, mirror chamber and cat intruder stressors were used. Tissues were isolated from the animal and homogenized for tissue antioxidant assay. Serum was collected for assays of urea and creatinine for the kidney function test using ELISA. Data collected were analyzed for Mean ± SEM using One Way ANOVA. The present study revealed that exposure of rats to different stressors reduced relative kidney weights but did not significantly alter serum creatinine concentration in the Wistar rats, although the concentrations were slightly increased compared to controls. Urea concentration was significantly (p < 0.05) increased in rats exposed to restraint and intruder stressors. Exposure to a mirror chamber stressor did not significantly alter urea concentration. Offspring from parents of stressed female rats exhibited a significant (p < 0.05) increase in serum urea level, minimal increase in serum creatinine levels. GSH and GST levels showed no significant difference when compared to control group, whereas, GPx were significantly (p < 0.05) decreased irrespective of the stressor applied. SOD activity were significantly (p < 0.05) reduced in the group exposed to restraint or cat intruder stressor. CAT activities were significantly (p < 0.05) reduced in the rats exposed to restraint or cat intruder stressor. In all, the different stress model altered the antioxidant capacity of the kidney tissues. Exposure of rats to a stressful condition of the different nature of stressor has the tendency of compromising the functional integrity of the kidney, thus, with the potency of complicating female renal function.
在环境中存在的各种应激源的作用下，肾脏的细胞完整性在稳态调节中不断受到氧化应激的损害。本研究的目的是评估不同应激源刺激对肾脏的影响。实验-1 使用了 24 只平均体重为 160-200g、年龄在 12-14 周的成年雌性大鼠，实验-2 使用了 12 只后代。使用了三种应激模型，即束缚、镜像室和猫入侵应激源。从动物身上分离组织并匀浆，用于组织抗氧化测定。收集血清，用于 ELISA 测定肾功能试验中的尿素和肌酐。使用 One Way ANOVA 对收集的数据进行均值 ± SEM 分析。本研究表明，暴露于不同应激源的大鼠的相对肾脏重量降低，但对 Wistar 大鼠的血清肌酐浓度没有显著影响，尽管与对照组相比，浓度略有升高。暴露于束缚和入侵应激源的大鼠的尿素浓度显著（p < 0.05）增加。暴露于镜像室应激源并未显著改变尿素浓度。来自应激雌性大鼠的后代的血清尿素水平显著（p < 0.05）增加，血清肌酐水平略有增加。与对照组相比，GSH 和 GST 水平没有显著差异，而无论应用何种应激源，GPx 均显著（p < 0.05）降低。暴露于束缚或猫入侵应激源的大鼠的 SOD 活性显著（p < 0.05）降低。暴露于束缚或猫入侵应激源的大鼠的 CAT 活性显著（p < 0.05）降低。总之，不同的应激模型改变了肾脏组织的抗氧化能力。暴露于不同性质的应激源会损害肾脏的功能完整性，从而有可能使女性的肾功能复杂化。
