Physiology, Shri BM Patil Medical College, Bijapur, Karnataka, India.
Anatomy, Shri BM Patil Medical College, Bijapur, India.
J Basic Clin Physiol Pharmacol. 2021 Mar 22;33(4):409-417. doi: 10.1515/jbcpp-2020-0106.
Dietary high fat possibly causes oxidative stress; also it makes deleterious effect on kidney functions and land up in lipotoxicity in renal tissue. Lipotoxicity is the pathological situation where lipid Peroxidation occurs and generates reactive oxygen species (ROS). Overproduction of ROS than antioxidant present in tissues cause oxidative stress. is found to be potential antioxidant that counteract oxidative stress and possibly maintain glomerular integrity.
Ethanolic extract of (ETO) was prepared and phytochemical analysis was done. Rats were divided into four groups, having six rats in each group as following; group 1-Control (20% fat); group 2 (ETO 100 mg/kg/b.wt); group 3 (30% fat) and group 4 (30% fat + ETO 100 mg/kg/b.wt). Dietary and ETO supplementation were continued for 21 days. Gravimetric, kidney functions (blood urea and serum creatinine) and oxidative stress markers like MDA, SOD and GSH were evaluated. Histopathological analysis was done on kidney along with measurement of glomerular integrity. Morphometrical analysis of glomerular integrity was evaluated by measuring glomerular length, width, glomerular area and Bowman's capsule radius. One way ANOVA was done for analysis of data.
Blood urea and serum creatinine levels were significantly higher in high fat fed rats indicating renal dysfunction. High fat diet showed significant increase in MDA, decrease in SOD and GSH in rats fed with high fat diet, which indicate generation of oxidative stress. Supplementation of ETO showed amelioratic effect against high fat diet induced renal dysfunction and oxidative stress. Histopathological findings were significantly corroborated with morphometrical analysis of glomerular integrity.
Ethanolic extracts of supplementation found to be beneficial against high fat induced renal alterations in terms of functions and architecture.
高脂肪饮食可能导致氧化应激；此外，它还会对肾脏功能造成有害影响，导致肾组织中的脂毒性。脂毒性是指脂质过氧化发生并产生活性氧（ROS）的病理情况。组织中产生的 ROS 超过抗氧化剂的量会导致氧化应激。 被发现是一种潜在的抗氧化剂，可以对抗氧化应激，并可能维持肾小球的完整性。
制备 的乙醇提取物，并进行植物化学分析。将大鼠分为四组，每组 6 只，如下所示：第 1 组-对照（20%脂肪）；第 2 组（ETO100mg/kg/b.wt）；第 3 组（30%脂肪）和第 4 组（30%脂肪+ETO100mg/kg/b.wt）。饮食和 ETO 补充持续 21 天。评估体重、肾功能（血尿素和血清肌酐）和氧化应激标志物如 MDA、SOD 和 GSH。对肾脏进行组织病理学分析，并测量肾小球完整性。通过测量肾小球长度、宽度、肾小球面积和鲍曼氏囊半径来评估肾小球完整性的形态计量学分析。采用单因素方差分析进行数据分析。
高脂肪喂养的大鼠血尿素和血清肌酐水平显著升高，表明肾功能障碍。高脂肪饮食显著增加了 MDA，降低了 SOD 和 GSH，这表明氧化应激的产生。ETO 的补充表现出对高脂肪饮食诱导的肾功能障碍和氧化应激的改善作用。组织病理学发现与肾小球完整性的形态计量学分析显著相符。
乙醇提取物的补充被发现对高脂肪引起的肾脏功能和结构改变有益。
