National Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
Anal Chem. 2023 Aug 29;95(34):12948-12955. doi: 10.1021/acs.analchem.3c02691. Epub 2023 Aug 17.
With the widespread use of drugs, drug-induced acute kidney injury (AKI) has become an increasingly serious health concern worldwide. Currently, early diagnosis of drug-induced AKI remains challenging because of the lack of effective biomarkers and noninvasive imaging tools. SO plays important physiological roles in living systems and is an important antioxidant for maintaining redox homeostasis. However, the relationship between SO (in water as SO/HSO) and drug-induced AKI remains largely unknown. Herein, we report the highly sensitive near-infrared fluorescence probe , which for the first time reveals the relationship between SO and drug-induced AKI. The probe responds to SO/HSO selectively and rapidly (within seconds) and shows a significant turn-on fluorescence at 710 nm with a large Stokes shift (125 nm). With these properties, the probe was successfully applied to detect SO in living cells and mice. Importantly, the probe can selectively target the kidneys, allowing for the detection of changes in the SO concentration in the kidneys. Based on this, was successfully used to detect cisplatin-induced AKI and revealed an increase in the SO levels. The results indicate that SO is a new biomarker for AKI and that is a powerful tool for studying and diagnosing drug-induced AKI.
随着药物的广泛使用，药物引起的急性肾损伤（AKI）已成为全球日益严重的健康问题。目前，由于缺乏有效的生物标志物和非侵入性成像工具，药物引起的 AKI 的早期诊断仍然具有挑战性。SO 在生命系统中发挥着重要的生理作用，是维持氧化还原平衡的重要抗氧化剂。然而，SO（以 SO/HSO 的形式存在于水中）与药物引起的 AKI 之间的关系在很大程度上尚不清楚。在此，我们报告了一种高灵敏度的近红外荧光探针 ，它首次揭示了 SO 与药物引起的 AKI 之间的关系。该探针对 SO/HSO 具有选择性和快速响应（在几秒钟内），并在 710nm 处显示出显著的开荧光，Stokes 位移大（125nm）。凭借这些特性，该探针成功地应用于活细胞和小鼠中 SO 的检测。重要的是，该探针可以选择性地靶向肾脏，从而可以检测肾脏中 SO 浓度的变化。基于这一点，成功地用于检测顺铂引起的 AKI，并显示 SO 水平升高。结果表明，SO 是 AKI 的一个新的生物标志物，而 是研究和诊断药物引起的 AKI 的有力工具。
