Navarro-Leyva Alicia, López-Angulo Gabriela, Delgado-Vargas Francisco, López-Valenzuela José Ángel
Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria, Culiacán, Sinaloa, México.
J Food Sci. 2023 Oct;88(10):4262-4274. doi: 10.1111/1750-3841.16744. Epub 2023 Aug 17.
Chickpea (ICC3761) protein hydrolysates have shown high in vitro antioxidant activity (AoxA) and antidiabetic potential. The aim of this study was to evaluate the in vivo activities (i.e., antioxidant, anti-inflammatory, hypoglycemic, and anti-hyperglycemic) of chickpea albumin hydrolysates (CAH) obtained with alcalase and pepsin-pancreatin (fractions ≤ 10 kDa). The CAH were analyzed for degree of hydrolysis (DH), electrophoretic and chromatographic profiles, and in vitro AoxA (2,2'-azino-bis(3-ethylbenzothiazolin)-6-sulfonic acid [ABTS], 2,2-diphenyl-1-pycrilhydrazyl [DPPH]). They were also evaluated for AoxA, anti-inflammatory and hypo- and anti-hyperglycemic activities in BALB-c mice. The DH was 20% for the alcalase CAH and 50% for the pepsin-pancreatin CAH, while the AoxA by ABTS (1 mg/mL) was 64.8% and 64.9% and by DPPH (5 mg/mL) was 48.0% and 31.1%. In the in vivo AoxA assay, mice of non-damaged control and those treated with both CAH showed similar alkaline phosphatase values, control and pepsin-pancreatin treated groups had similar malondialdehyde levels, while treated and non-damaged control groups had higher glutathione levels than the damaged control. Liver histopathology revealed that the pepsin-pancreatin CAH mitigated most of the pathological changes associated with the induced oxidative damage. Both CAH (2 mg/ear) reduced croton oil-induced ear edema in mice. The α-glucosidase inhibition of CAH (100 mg/mL) was 31.1% (alcalase) and 52.4% (pepsin-pancreatin). Mice treated with alcalase CAH (100 mg/mL) and glibenclamide exhibited similar hypoglycemic activities, whereas only those treated with the pepsin-pancreatin CAH (200 mg/kg body weight) showed anti-hyperglycemic activity. The results indicate that CAH can be used as a source of bioactive peptides with antioxidant, anti-inflammatory, hypoglycemic, and anti-hyperglycemic activities.
鹰嘴豆（ICC3761）蛋白水解物已显示出较高的体外抗氧化活性（AoxA）和抗糖尿病潜力。本研究的目的是评估用碱性蛋白酶和胃蛋白酶 - 胰蛋白酶获得的鹰嘴豆白蛋白水解物（CAH，组分≤10 kDa）的体内活性（即抗氧化、抗炎、降血糖和抗高血糖活性）。分析了CAH的水解度（DH）、电泳和色谱图谱以及体外AoxA（2,2'-联氮 - 双 -（3 - 乙基苯并噻唑啉 - 6 - 磺酸）[ABTS]、2,2 - 二苯基 - 1 - 苦基肼[DPPH]）。还评估了它们在BALB - c小鼠中的AoxA、抗炎以及降血糖和抗高血糖活性。碱性蛋白酶CAH的DH为20%，胃蛋白酶 - 胰蛋白酶CAH的DH为50%，而ABTS法（1 mg/mL）测定的AoxA分别为64.8%和64.9%，DPPH法（5 mg/mL）测定的分别为48.0%和31.1%。在体内AoxA测定中，未受损对照组小鼠和两种CAH处理组小鼠的碱性磷酸酶值相似，对照组和胃蛋白酶 - 胰蛋白酶处理组的丙二醛水平相似，而处理组和未受损对照组的谷胱甘肽水平高于受损对照组。肝脏组织病理学显示，胃蛋白酶 - 胰蛋白酶CAH减轻了与诱导的氧化损伤相关的大部分病理变化。两种CAH（2 mg/耳）均减轻了巴豆油诱导的小鼠耳部水肿。CAH（100 mg/mL）对α - 葡萄糖苷酶的抑制率分别为31.1%（碱性蛋白酶）和52.4%（胃蛋白酶 - 胰蛋白酶）。用碱性蛋白酶CAH（100 mg/mL）处理的小鼠和用格列本脲处理小鼠的降血糖活性相似，而只有用胃蛋白酶 - 胰蛋白酶CAH（200 mg/kg体重）处理的小鼠表现出抗高血糖活性。结果表明CAH可作为具有抗氧化、抗炎、降血糖和抗高血糖活性的生物活性肽来源。
