Kang Lingzhi, Zhou Ying, Chen Xifang, Yue Zhilian, Liu Xiao, Baker Chris, Wallace Gordon G
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Wollongong, NSW, 2522, Australia.
Department of Dermatology, St Vincent's Hospital Melbourne, Melbourne, VIC, 3065, Australia.
Macromol Biosci. 2023 Dec;23(12):e2300220. doi: 10.1002/mabi.202300220. Epub 2023 Aug 23.
The development of biomimetic structures with integrated extracellular matrix (ECM) components represents a promising approach to biomaterial fabrication. Here, an artificial ECM, comprising the structural protein collagen I and elastin (ELN), as well as the glycosaminoglycan hyaluronan (HA), is reported. Specifically, collagen and ELN are electrochemically aligned to mimic the compositional characteristics of the dermal matrix. HA is incorporated into the electro-compacted collagen-ELN matrices via adsorption and chemical immobilization, to give a final composition of collagen/ELN/HA of 7:2:1. This produces a final collagen/ELN/hyaluronic acid scaffold (CEH) that recapitulates the compositional feature of the native skin ECM. This study analyzes the effect of CEH composition on the cultivation of human dermal fibroblast cells (HDFs) and immortalized human keratinocytes (HaCaTs). It is shown that the CEH scaffold supports dermal regeneration by promoting HDFs proliferation, ECM deposition, and differentiation into myofibroblasts. The CEH scaffolds are also shown to support epidermis growth by supporting HaCaTs proliferation, differentiation, and stratification. A double-layered epidermal-dermal structure is constructed on the CEH scaffold, further demonstrating its ability in supporting skin cell function and skin regeneration.
开发具有整合细胞外基质（ECM）成分的仿生结构是生物材料制造的一种有前景的方法。在此，报道了一种人工ECM，其包含结构蛋白I型胶原蛋白和弹性蛋白（ELN）以及糖胺聚糖透明质酸（HA）。具体而言，胶原蛋白和ELN通过电化学方式排列以模拟真皮基质的组成特征。HA通过吸附和化学固定掺入电压实的胶原蛋白-ELN基质中，得到最终胶原蛋白/ELN/HA组成为7:2:1。这产生了一种最终的胶原蛋白/ELN/透明质酸支架（CEH），其概括了天然皮肤ECM的组成特征。本研究分析了CEH组成对人真皮成纤维细胞（HDFs）和永生化人角质形成细胞（HaCaTs）培养的影响。结果表明，CEH支架通过促进HDFs增殖、ECM沉积以及向肌成纤维细胞分化来支持真皮再生。CEH支架还通过支持HaCaTs增殖、分化和分层来支持表皮生长。在CEH支架上构建了双层表皮-真皮结构，进一步证明了其支持皮肤细胞功能和皮肤再生的能力。
