Bankhurst A D, Anderson R E, Cram L S, Horan P H, Warner N L
Aust J Exp Biol Med Sci. 1978 Oct;56(5):571-7. doi: 10.1038/icb.1978.63.
The purpose of the present study was to determine whether at least some splenic B lymphocytes can switch from the synthesis of one isotype of immunoglobulin to another during B cell differentiation. The experimental system invovled the transfer of characterized cell suspensions between allotype congenic strains of mice followed by analysis in the recipient for donor type immunoglobulin production. Donor splenic lymphocytes were incubated with specific fluorescent labelled anti-mu antiserum and passed through the Los Alamos fluorescence-activated cell sorter; mu-depleted cell suspensions were transferred into sublethally irradiated congenic recipients and the amount of donor type immunoglobulin of IgG2 type was measured at weekly intervals. The results demonstrated taht at least some cell bearing membrane bound IgM can differentiate in vivo into IgG2-secreting cells, although not all IgG2-secreting cells have been recently derived from IgM positive precursors.
本研究的目的是确定在B细胞分化过程中，至少一些脾脏B淋巴细胞是否能从一种免疫球蛋白同种型的合成转换为另一种。实验系统包括在小鼠的同种异型同基因品系之间转移特征明确的细胞悬液，然后在受体中分析供体型免疫球蛋白的产生。将供体脾脏淋巴细胞与特异性荧光标记的抗μ抗血清孵育，然后通过洛斯阿拉莫斯荧光激活细胞分选仪；将去除μ的细胞悬液转移到亚致死剂量照射的同基因受体中，每周测量一次供体型IgG2型免疫球蛋白的量。结果表明，至少一些带有膜结合IgM的细胞在体内可分化为分泌IgG2的细胞，尽管并非所有分泌IgG2的细胞最近都来源于IgM阳性前体。
