Chen Guifang, Li Huijie, Gao Yunhua, Zhao Hang, Yang Jiayi, Dong Lianhua
Center for Advanced Measurement Science, National Institute of Metrology, Beijing, China.
Shenzhen Institute for Technology Innovation, National Institute of Metrology, Shenzhen, China.
Foodborne Pathog Dis. 2023 Oct;20(10):453-459. doi: 10.1089/fpd.2023.0011. Epub 2023 Aug 17.
Coinfection with human adenovirus (HAdV) and SARS-CoV-2 has been associated with acute hepatitis in children with unknown etiology. Similar cases have been reported in many countries, and HAdV 40 and HAdV 41 have been identified. The quantification method is established based on digital PCR (dPCR) for HAdV 40/41, which is more convenient for low-concentration virus detection. The limit of detections of HAdV 40/41 dPCR were 4 and 5 copies/μL. Pseudovirus reference material (RM) that contains the highly conserved gene was developed and quantified with the dPCR method. The assigned values with expanded uncertainty were (1.43 ± 0.35) × 10 copies/μL for HAdV 40 RM and (1.21 ± 0.28) × 10 copies/μL for HAdV 41 RM. The values could be reproduced on multiple platforms. The dPCR method and pseudovirus RMs contribute to the improved accuracy of HAdV 40/41 detection, which is crucial for clinical diagnosis.
人腺病毒（HAdV）与新型冠状病毒（SARS-CoV-2）的合并感染与病因不明的儿童急性肝炎有关。许多国家都报告了类似病例，并且已鉴定出腺病毒40型（HAdV 40）和腺病毒41型（HAdV 41）。基于数字PCR（dPCR）建立了针对HAdV 40/41的定量方法，该方法更便于检测低浓度病毒。HAdV 40/41 dPCR的检测限分别为4和5拷贝/微升。开发了包含高度保守基因的假病毒参考物质（RM），并用dPCR方法进行定量。对于HAdV 40 RM，具有扩展不确定度的赋值为（1.43±0.35）×10拷贝/微升，对于HAdV 41 RM为（1.21±0.28）×10拷贝/微升。这些值可以在多个平台上重现。dPCR方法和假病毒RM有助于提高HAdV 40/41检测的准确性，这对临床诊断至关重要。
