Kocher O, Gabbiani G
Hum Pathol. 1986 Sep;17(9):875-80. doi: 10.1016/s0046-8177(86)80637-2.
The distribution of actin, vimentin, desmin, and tropomyosin was studied in the media of the human aorta and femoral and coronary arteries, as well as in atheromatous plaques from the same arteries, by means of immunofluorescence, densitometric analysis of sodium dodecylsulfate-polyacrylamide gel electrophoresis, and bidimensional gel electrophoresis. The proportions of desmin-containing cells varied in the media of different arteries; 4 per cent of the cells in the aorta, 11 per cent in the coronary artery, and 37 per cent in the femoral artery contained desmin. In fibrous atheromatous plaques, independently of the artery, desmin-containing cells were almost absent, but they reappeared in complicated lesions. The content of vimentin per smooth muscle cell increased in fibrous atheromatous plaques, whereas the content of actin and tropomyosin was less than in normal media. Moreover, the alpha-actin predominance observed in the media was transformed to beta-actin predominance in the atheromatous plaques. These cytoskeletal changes provide new, possibly useful, biochemical markers for the characterization of smooth muscle cells during early and advanced phases of atheroma formation.
通过免疫荧光、十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳的光密度分析和双向凝胶电泳，研究了肌动蛋白、波形蛋白、结蛋白和原肌球蛋白在人主动脉、股动脉和冠状动脉中层以及这些动脉粥样斑块中的分布。含结蛋白的细胞比例在不同动脉中层有所不同；主动脉中层4%的细胞、冠状动脉中层11%的细胞以及股动脉中层37%的细胞含有结蛋白。在纤维性动脉粥样斑块中，无论何种动脉，含结蛋白的细胞几乎不存在，但在复杂病变中又重新出现。纤维性动脉粥样斑块中每个平滑肌细胞波形蛋白的含量增加，而肌动蛋白和原肌球蛋白的含量低于正常中层。此外，中层观察到的α - 肌动蛋白优势在动脉粥样斑块中转变为β - 肌动蛋白优势。这些细胞骨架变化为动脉粥样硬化形成早期和晚期平滑肌细胞的特征化提供了新的、可能有用的生化标志物。
