嗜水气单胞菌溶血素增强了鲫鱼血清酶活性，并调节了脾脏的转录反应。
Hemolysin from Aeromonas hydrophila enhances the host's serum enzyme activity and regulates transcriptional responses in the spleen of Cyprinus rubrofuscus.
机构信息
School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China; College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China.
出版信息
Ecotoxicol Environ Saf. 2023 Sep 15;263:115375. doi: 10.1016/j.ecoenv.2023.115375. Epub 2023 Aug 15.
Aeromonas hydrophila is a conditional pathogen impacting public hygiene and safety. Hemolysin is a virulence factor of Aeromonas hydrophila that causes erythrocyte hemolysis, yet its transcriptional response to Cyprinus rubrofuscus remains unknown. Our investigation confirmed the hemolysis of hemolysin from A. hydrophila. Serum enzyme activity was evaluated weekly after C. rubrofuscus were immunized with hemolysin Ahh1. The results showed that the hemolysin enhances the serum superoxide dismutase (SOD), lysozyme (LZM), and catalase (CAT) activity, which reached a maximum on day 14. To elucidate the molecular interaction between hemolysin from A. hydrophila and the host, we performed transcriptome sequencing on the spleen of C. rubrofuscus 14 days post hemolysin infection. The total number of clean reads was 41.37 Gb, resulting in 79,832 unigenes with an N50 length of 1863 bp. There were 1982 significantly differentially expressed genes (DEGs), including 1083 upregulated genes and 899 downregulated genes. Transcript levels of the genes, such as LA6BL, CD2, and NLRC5, were significantly downregulated, while those of IL11, IL1R2, and IL8 were dramatically upregulated. The DEGs were mainly enriched in the immune disease, viral protein interaction with cytokine and cytokine receptor, and toll-like receptor pathways, suggesting that hemolysin stimulation can activate the transcriptional responses. RT-qPCR experiments results of seven genes, IL-8, STAT2, CTSK, PRF1, CXCL9, TLR5, and SACS, showed that their expression was highly concordant with RNA-seq data. We clarified for the first time the key genes and signaling pathways response to hemolysin from A. hydrophila, which offers strategies for treating and preventing diseases.
嗜水气单胞菌是一种对公共卫生和安全有影响的条件致病菌。溶血素是嗜水气单胞菌的一种毒力因子，可导致红细胞溶血，但它对红鲫的转录反应尚不清楚。我们的研究证实了嗜水气单胞菌溶血素的溶血作用。用溶血素 Ahh1 免疫红鲫后，每周评估血清酶活性。结果表明，溶血素增强了血清中超氧化物歧化酶（SOD）、溶菌酶（LZM）和过氧化氢酶（CAT）的活性，第 14 天达到最大值。为了阐明嗜水气单胞菌溶血素与宿主之间的分子相互作用，我们对溶血素感染红鲫 14 天后的脾脏进行了转录组测序。总清洁读取数为 41.37Gb，产生了 79832 个长度为 1863bp 的非重叠基因。有 1982 个显著差异表达基因（DEGs），包括 1083 个上调基因和 899 个下调基因。LA6BL、CD2 和 NLRC5 等基因的转录水平显著下调，而 IL11、IL1R2 和 IL8 等基因的转录水平则显著上调。DEGs 主要富集在免疫性疾病、病毒蛋白与细胞因子和细胞因子受体的相互作用以及 Toll 样受体途径，表明溶血素刺激可以激活转录反应。七个基因（IL-8、STAT2、CTSK、PRF1、CXCL9、TLR5 和 SACS）的 RT-qPCR 实验结果表明，它们的表达与 RNA-seq 数据高度一致。我们首次阐明了红鲫对嗜水气单胞菌溶血素的关键基因和信号通路反应，为治疗和预防疾病提供了策略。
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