DZ1 蜡样芽胞杆菌噬菌体的分离与鉴定及其在食品中的应用。
Isolation and characterization of Bacillus cereus bacteriophage DZ1 and its application in foods.
机构信息
Department of Food Science & Engineering, Institute of Food Safety & Nutrition, Jinan University, Guangzhou 510632, China; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Department of Food Science & Engineering, Institute of Food Safety & Nutrition, Jinan University, Guangzhou 510632, China.
出版信息
Food Chem. 2024 Jan 15;431:137128. doi: 10.1016/j.foodchem.2023.137128. Epub 2023 Aug 11.
Bacillus cereus is a pathogenic bacterium that causes food contamination, resulting in food poisoning such as diarrhea and emesis. Therefore, it is crucial to develop effective strategies to control this bacterium. In this study, we isolated and characterized a novel B. cereus phage, named DZ1. Morphological and genomic analyses revealed that phage DZ1 is a new species belonging to the Andromedavirus genus. Phage DZ1 was tolerant to a wide range of pH values (5-9), temperatures (4-55 ℃), and high concentrations of NaCl solution (1000 mM). B. cereus with 21 different sequence types (STs) can be lysed by phage DZ1. Importantly, phage DZ1 inhibited B. cereus growth in spiked rice substrates or milk up to 36 and 72 h, respectively, with suppression of 3 log. Therefore, phage DZ1 is a useful biocontrol agent for the control of B. cereus in the food industry.
蜡样芽胞杆菌是一种致病菌，可导致食物污染，引发腹泻和呕吐等食物中毒。因此，开发有效控制该细菌的策略至关重要。本研究分离并鉴定了一株新型蜡样芽胞杆菌噬菌体，命名为 DZ1。形态学和基因组分析表明，噬菌体 DZ1 是属于 Andromedavirus 属的一个新种。噬菌体 DZ1 可耐受广泛的 pH 值（5-9）、温度（4-55℃）和高浓度 NaCl 溶液（1000 mM）。噬菌体 DZ1 可以裂解 21 种不同序列类型（ST）的蜡样芽胞杆菌。重要的是，噬菌体 DZ1 可抑制污染米饭基质或牛奶中蜡样芽胞杆菌的生长，抑制率分别高达 3 个对数级，分别为 36 和 72 h。因此，噬菌体 DZ1 是一种可用于控制食品工业中蜡样芽胞杆菌的生物防治剂。
Zotero 插件