School of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin 300072, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
Food Chem. 2024 Jan 15;431:137058. doi: 10.1016/j.foodchem.2023.137058. Epub 2023 Jul 29.
A magnetic solid-phase extraction (MSPE) protocol using novel Urea-based magnetic covalent organic framework coupled with liquid chromatography-mass spectrometry was developed for the detection of coumarins in food samples (soft drink, biscuit and sesame paste). This adsorbent was synthesized through atom economic polymerization of tetrakis(4-aminophenyl) methane and 1,4-phenylene diisocyanate, which was successfully verified by a series of techniques. Major parameters influencing MSPE efficiency were optimized. This protocol had some advantages, such as organic-reagent-saving (2.0 mL), easy operating, short extraction time, and high repeatability (8 times). The established method exhibited superior linearity (R ≥ 0.999) and the limits of detection ranging from 1.0 to 5.0 µg/kg. The recoveries of coumarin and its derivatives ranged from 73.8% to 113.5% and both intra- and inter-day precision were less than 15%. These data indicate the protocol is a highly promising alternative for coumarin extraction and enrichment.
建立了一种基于脲基的新型磁性共价有机框架固相萃取（MSPE）与液相色谱-质谱联用检测食品样品（软饮料、饼干和芝麻酱）中香豆素的方法。该吸附剂是通过四（4-氨基苯基）甲烷和 1,4-亚苯基二异氰酸酯的原子经济聚合合成的，通过一系列技术成功验证。优化了影响 MSPE 效率的主要参数。该方法具有有机溶剂用量少（2.0 mL）、操作简单、提取时间短、重复性好（8 次）等优点。所建立的方法具有优异的线性（R≥0.999）和检测限范围为 1.0 至 5.0 µg/kg。香豆素及其衍生物的回收率在 73.8%至 113.5%之间，日内和日间精密度均小于 15%。这些数据表明，该方法是香豆素提取和富集的一种很有前途的替代方法。
