Tuncer Sibel Çiğdem, Küçükler Sefa, Gür Cihan, Aygörmez Serpil, Kandemir Fatih Mehmet
Department of Medical Biochemistry, Faculty of Medicine, Aksaray University, Aksaray, Türkiye.
Department of Veterinary Biochemistry, Faculty of Veterinary, Atatürk University, Erzurum, Türkiye.
Mol Biol Rep. 2023 Oct;50(10):8305-8318. doi: 10.1007/s11033-023-08715-8. Epub 2023 Aug 17.
Cadmium (Cd) is a strong toxic agent and causes serious damage to testicular tissues. Chrysin (CHR) is a natural flavonoid with many effective properties, especially antioxidant, anti-inflammatory and anti-apoptotic properties. The current study describes new evidence for the ameliorative effects of CHR on oxidative stress, apoptosis, autophagy and inflammation pathways in Cd-induced testicular tissue toxicity.
Thirty-five male Wistar rats were divided into five groups, control, Cd, CHR, Cd + CHR25, and Cd + CHR50. Cd was administered alone at a dose of 25 mg/kg body weight or in combination with CHR 25 mg/kg and CHR 50 mg/kg for 7 days. Cd and CHR were administered orally. Biochemical, molecular, and histological methods were used to investigate inflammation, apoptosis, autophagy, and oxidant pathways in testicular tissue.
Cd increased lipid peroxidation, JAK-2/STAT-3 levels, inflammation-related NF-κB, TNF-α, IL-1β, IL-6, COX-2, and iNOS levels, AKT-2, FOXO1, Bax, Apaf-1 and Caspase-3 levels, autophagic Beclin-1, LC3A and LC3B. The Cd also caused a decrease in the activities of antioxidant enzymes and GSH levels, antiapoptotic Bcl-2 levels. CHR, on the other hand, had the opposite effect of all these Cd-induced changes.
Overall, the data of this study indicate that testicular damage associated with Cd toxicity could be ameliorated by CHR administration.
镉（Cd）是一种强毒性物质，会对睾丸组织造成严重损害。白杨素（CHR）是一种具有多种有效特性的天然黄酮类化合物，尤其是抗氧化、抗炎和抗凋亡特性。本研究描述了白杨素对镉诱导的睾丸组织毒性中氧化应激、凋亡、自噬和炎症途径改善作用的新证据。
将35只雄性Wistar大鼠分为五组，即对照组、镉组、白杨素组、镉+25mg/kg白杨素组和镉+50mg/kg白杨素组。单独给予镉，剂量为25mg/kg体重，或与25mg/kg和50mg/kg白杨素联合给药7天。镉和白杨素通过口服给药。采用生化、分子和组织学方法研究睾丸组织中的炎症、凋亡、自噬和氧化途径。
镉增加了脂质过氧化、JAK-2/STAT-3水平、炎症相关的NF-κB、TNF-α、IL-1β、IL-6、COX-2和iNOS水平、AKT-2、FOXO1、Bax、Apaf-1和Caspase-3水平、自噬相关的Beclin-1、LC3A和LC3B。镉还导致抗氧化酶活性和谷胱甘肽水平降低，抗凋亡的Bcl-2水平降低。另一方面，白杨素对所有这些镉诱导的变化具有相反的作用。
总体而言，本研究数据表明，给予白杨素可改善与镉毒性相关的睾丸损伤。
