Guillamón-Vivancos Teresa, Vandael Dorien, Torres Daniel, López-Bendito Guillermina, Martini Francisco J
Instituto de Neurociencias de Alicante, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas (UMH-CSIC), Sant Joan d'Alacant, Spain.
Front Neurosci. 2023 Aug 1;17:1210199. doi: 10.3389/fnins.2023.1210199. eCollection 2023.
Calcium imaging is commonly used to visualize neural activity . In particular, mesoscale calcium imaging provides large fields of view, allowing for the simultaneous interrogation of neuron ensembles across the neuraxis. In the field of Developmental Neuroscience, mesoscopic imaging has recently yielded intriguing results that have shed new light on the ontogenesis of neural circuits from the first stages of life. We summarize here the technical approaches, basic notions for data analysis and the main findings provided by this technique in the last few years, with a focus on brain development in mouse models. As new tools develop to optimize calcium imaging , basic principles of neural development should be revised from a mesoscale perspective, that is, taking into account widespread activation of neuronal ensembles across the brain. In the future, combining mesoscale imaging of the dorsal surface of the brain with imaging of deep structures would ensure a more complete understanding of the construction of circuits. Moreover, the combination of mesoscale calcium imaging with other tools, like electrophysiology or high-resolution microscopy, will make up for the spatial and temporal limitations of this technique.
钙成像常用于可视化神经活动。特别是，中尺度钙成像提供了大视野，允许同时对整个神经轴的神经元群体进行研究。在发育神经科学领域，中尺度成像最近产生了有趣的结果，为生命最初阶段神经回路的个体发生提供了新的线索。我们在此总结了过去几年该技术的技术方法、数据分析的基本概念以及主要发现，重点是小鼠模型中的脑发育。随着优化钙成像的新工具不断发展，神经发育的基本原理应从中尺度角度进行修订，即考虑到全脑神经元群体的广泛激活。未来，将脑背表面的中尺度成像与深部结构的成像相结合，将确保对回路构建有更全面的理解。此外，中尺度钙成像与其他工具（如电生理学或高分辨率显微镜）的结合，将弥补该技术的空间和时间限制。
