Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology and Agro-Bioengineering, CICMEAB, College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, Guizhou Province, China.
Acta Biochim Pol. 2023 Aug 17;70(3):561-566. doi: 10.18388/abp.2020_6533.
MicroRNAs (miRNAs) are major players in cellular responses to xenobiotic compounds and toxins. However, the role of miRNAs in pyrethroid pesticide-induced cancer progression remains unclear. This study aimed to investigate the function of miR-96-5p in permethrin-induced proliferation and migration in breast cancer cells. In our study, the expression of miR-96-5p was upregulated in permethrin-treated MCF-7 cells. MiR-96-5p promoted MCF-7 cell proliferation and migration, accompanied bychanges in the expression of proteins involved in cell proliferation, migration, and apoptosis. Homeobox A5 (HOXA5) was identified as a direct target of miR-96-5p. HOXA5 silencing had the opposite effects with miR-96-5p inhibition. In conclusion, these results suggest that miR-96-5p is involved in permethrin-promoted proliferation and migration of breast cancer cells by targeting HOXA5.
微小 RNA（miRNAs）是细胞对外源化合物和毒素反应的主要参与者。然而，miRNAs 在拟除虫菊酯类农药诱导的癌症进展中的作用尚不清楚。本研究旨在探讨 miR-96-5p 在氯菊酯诱导的乳腺癌细胞增殖和迁移中的功能。在我们的研究中，miR-96-5p 在氯菊酯处理的 MCF-7 细胞中表达上调。miR-96-5p 促进 MCF-7 细胞增殖和迁移，伴随着参与细胞增殖、迁移和凋亡的蛋白表达的变化。同源盒 A5（HOXA5）被鉴定为 miR-96-5p 的直接靶标。HOXA5 沉默与 miR-96-5p 抑制具有相反的效果。总之，这些结果表明，miR-96-5p 通过靶向 HOXA5 参与氯菊酯促进乳腺癌细胞的增殖和迁移。
