环状 POLA2 介导的 miR-138-5p/SEMA4C 轴影响结肠癌细胞活性。
Circ-POLA2-mediated miR-138-5p/SEMA4C axis affects colon cancer cell activities.
机构信息
Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China.
Practical Teaching Skills Center, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China.
出版信息
Acta Biochim Pol. 2023 Aug 17;70(3):517-523. doi: 10.18388/abp.2020_6457.
This study aimed to investigate the mechanism of circ-POLA2 in colon cancer (CC). Circ-POLA2, miR-138-5p, and SEMA4C levels in CC tissues and cells were recorded. The influences mediated by circ-POLA2, miR-138-5p or SEMA4C on cell proliferation, migration, invasion, and apoptosis were determined. The feedback loop of circ-POLA2/miR-138-5p/SEMA4C was surveyed. As measured, circ-POLA2 and SEMA4C were highly expressed, while miR-138-5p was poorly expressed. Meanwhile, circ-POLA2 could mediate SEMA4C through miR-138-5p targeting. Circ-POLA2 knockdown caused the blockade for cell activities, but this effect was alleviated by miR-138-5p inhibition or SEMA4C overexpression. Overall, circ-POLA2 is tumorigenic for CC through miR-138-5p/SEMA4C axis, which may provide a promising molecular target for CC therapy.
本研究旨在探讨环状 RNA 聚合酶 II（circ-POLA2）在结肠癌（CC）中的作用机制。记录 CC 组织和细胞中的 circ-POLA2、miR-138-5p 和 SEMA4C 水平。确定 circ-POLA2、miR-138-5p 或 SEMA4C 对细胞增殖、迁移、侵袭和凋亡的影响。还调查了 circ-POLA2/miR-138-5p/SEMA4C 的反馈回路。结果表明，circ-POLA2 和 SEMA4C 表达上调，而 miR-138-5p 表达下调。同时，circ-POLA2 可以通过 miR-138-5p 靶向来介导 SEMA4C。circ-POLA2 敲低导致细胞活性受阻，但这种作用可以通过 miR-138-5p 抑制或 SEMA4C 过表达来缓解。总之，circ-POLA2 通过 miR-138-5p/SEMA4C 轴促进 CC 的发生，这可能为 CC 的治疗提供一个有前途的分子靶点。
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