National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
J Chromatogr A. 2023 Sep 27;1707:464290. doi: 10.1016/j.chroma.2023.464290. Epub 2023 Aug 16.
An ion chromatography (IC)-tandem mass spectrometry (MS/MS) method to analyze nerve agent degradation products in human urine was developed. Six degradation products of conventional nerve agents and six Novichok agent degradation products were analyzed simultaneously despite their differences in hydrophilicity and acidity. Using ammonium regeneration solution improved the peak shapes greatly compared with the results obtained with the ordinary IC-MS/MS configuration. For urine samples, a simple pretreatment method of dilution with water and ultrafiltration was used. The detection limits of the nerve agent degradation products were sufficiently low (10-250 ng/mL) and the calibration curves showed acceptable linearity. Due to the absence of a derivatization step, throughput was higher than for our previous derivatization-liquid chromatography-MS/MS method.
建立了一种离子色谱（IC）-串联质谱（MS/MS）法，用于分析人尿液中的神经性毒剂降解产物。尽管常规神经性毒剂降解产物和六种 Novichok 制剂降解产物在亲水性和酸度上存在差异，但仍可同时对其进行分析。与普通 IC-MS/MS 配置相比，使用氨再生溶液可大大改善峰形。对于尿液样本，采用了简单的预处理方法，即用去离子水稀释和超滤。神经性毒剂降解产物的检测限足够低（10-250ng/mL），校准曲线显示出可接受的线性。由于无需衍生化步骤，因此与我们之前的衍生化-液相色谱-MS/MS 方法相比，该方法的通量更高。
