Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat 13109, Kuwait.
Faculty of Medicine Research Core Facility, Kuwait University, P.O Box 24923, Safat 13110, Kuwait.
Food Chem. 2024 Jan 15;431:137154. doi: 10.1016/j.foodchem.2023.137154. Epub 2023 Aug 12.
The aim of this study was to develop vitamin D (VD) and iron (Fe) blended granules using Neusilin® US2 as an excipient. A central composite design of experiments was used for the continuous manufacturing process, considering VD and iron as independent variables and the bulk density, flow index, oil holding capacity, and color difference as response variables. The addition of VD had a significant effect on the powder flow properties. The X-ray diffraction and Scanning electron microscopy-energy dispersive X-ray analysis validated the presence of VD and Fe in the granules, whereas the variations in porosity and roughness were demonstrated by tomography and atomic force microscopy, respectively. The in vitro cellular uptake profile confirmed the absorption of VD in the breast cancer cell line MCF-7 with apparent apoptosis. These results could help in scaling up the process from laboratory to pilot scale in twin-screw granulation and boost the intervention of VitD/iron deficiencies.
本研究旨在开发以 Neusilin® US2 为赋形剂的维生素 D（VD）和铁（Fe）混合颗粒。采用中心复合设计实验对连续制造工艺进行研究，以 VD 和 Fe 作为独立变量，以堆密度、流动指数、持油能力和色差作为响应变量。VD 的添加对粉末流动性能有显著影响。X 射线衍射和扫描电子显微镜-能谱分析验证了颗粒中存在 VD 和 Fe，而孔隙率和粗糙度的变化分别通过断层扫描和原子力显微镜来证明。体外细胞摄取曲线证实了维生素 D 在乳腺癌细胞系 MCF-7 中的吸收，并伴有明显的细胞凋亡。这些结果有助于将实验室规模的工艺放大到双螺杆造粒的中试规模，并促进 VitD/铁缺乏症的干预。
