非理性方法优化 CRISPR-Cas9 复合物的靶向性。
A non-rational approach to optimized targeting of CRISPR-Cas9 complexes.
机构信息
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Rochester, MN 55905, USA.
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Rochester, MN 55905, USA.
出版信息
Cell Chem Biol. 2023 Aug 17;30(8):855-857. doi: 10.1016/j.chembiol.2023.07.012.
Abstract
In this issue of Cell Chemical Biology, Bush et al. report an in vitro selection method for optimizing CRISPR-Cas9 single-guide RNAs. This approach may be useful in targeting previously intractable genomic sequences. The results also provide insights into which positions in single-guide RNAs are most amenable to modification.
摘要
在本期《细胞化学生物学》中，Bush 等人报道了一种优化 CRISPR-Cas9 单引导 RNA 的体外选择方法。这种方法在靶向以前难以处理的基因组序列时可能很有用。研究结果还深入了解了单引导 RNA 中哪些位置最适合进行修饰。
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本文引用的文献
1
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2
CRISPR-Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing.CRISPR-Cas9：从发现到应用于基因组编辑的伦理考量简史。
Biochemistry (Mosc). 2022 Aug;87(8):777-788. doi: 10.1134/S0006297922080090.
3
Pioneers of revolutionary CRISPR gene editing win chemistry Nobel.革命性CRISPR基因编辑技术的先驱者荣获诺贝尔化学奖。
Nature. 2020 Oct;586(7829):346-347. doi: 10.1038/d41586-020-02765-9.
4
Cas9 Cleavage of Viral Genomes Primes the Acquisition of New Immunological Memories.Cas9 切割病毒基因组可诱导新免疫记忆的获得。
Cell Host Microbe. 2019 Oct 9;26(4):515-526.e6. doi: 10.1016/j.chom.2019.09.002. Epub 2019 Oct 1.
5
Functional Insights Revealed by the Kinetic Mechanism of CRISPR/Cas9.CRISPR/Cas9 的动力学机制揭示的功能见解。
J Am Chem Soc. 2018 Feb 28;140(8):2971-2984. doi: 10.1021/jacs.7b13047. Epub 2018 Feb 19.
6
The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems.II 型 CRISPR-Cas 免疫系统的 tracrRNA 和 Cas9 家族。
RNA Biol. 2013 May;10(5):726-37. doi: 10.4161/rna.24321. Epub 2013 Apr 5.
7
Multiplex genome engineering using CRISPR/Cas systems.利用 CRISPR/Cas 系统进行多重基因组工程。
Science. 2013 Feb 15;339(6121):819-23. doi: 10.1126/science.1231143. Epub 2013 Jan 3.
8
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10
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