Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing.
作者信息
He Yubing, Zhang Tao, Yang Ning, Xu Meilian, Yan Lang, Wang Lihao, Wang Rongchen, Zhao Yunde
机构信息
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
出版信息
J Genet Genomics. 2017 Sep 20;44(9):469-472. doi: 10.1016/j.jgg.2017.08.003. Epub 2017 Aug 24.
Abstract
摘要
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High-throughput functional genomics using CRISPR-Cas9.使用CRISPR-Cas9的高通量功能基因组学。
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