C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus, Surat 394 350, Gujarat, India.
J Appl Microbiol. 2023 Aug 1;134(8). doi: 10.1093/jambio/lxad189.
The aim of this study was to explore the decolourization and bioremediation ability of non-encapsulated and encapsulated Pseudomonas aeruginosa (strain KBN 12) against the azo dye brilliant blue (BB).
Six efficient BB dye-decolourizing bacteria were isolated from textile dye effluent. The most efficient free cells of P. aeruginosa KBN 12 along with the optimized conditions such as carbon source (maltose: 5 g L-1), and nitrogen source (ammonium chloride: 4 g L-1) at pH 6 at 37°C decolourized 72.69% of BB dye aerobically after 9 days of incubation under static conditions. Encapsulated (calcium alginate) P. aeruginosa KBN 12 decolourized 87.67% of BB dye aerobically after 9 days of incubation under the same optimized conditions. Fourier-transform infrared spectroscopy (FTIR) and gas chromatography (GC) analysis of the chemical structure of BB dye after decolourization found changes in functional and chemical groups. Phytotoxicity and soil respiration enzyme assays revealed that the decolourized dye or dye products were less toxic than the pure BB dye.
The encapsulation of P. aeruginosa KBN 12 proved to be an effective method for BB dye decolourization or remediation.
本研究旨在探索非包裹和包裹的铜绿假单胞菌（菌株 KBN12）对偶氮染料亮蓝（BB）的脱色和生物修复能力。
从纺织染料废水中分离出 6 株高效的 BB 染料脱色细菌。最有效的游离细胞铜绿假单胞菌 KBN12 与优化条件（碳源：麦芽糖 5g/L-1，氮源：氯化铵 4g/L-1，pH6，37°C）下，在静置条件下培养 9 天，可将 BB 染料有氧脱色 72.69%。在相同的优化条件下，包埋（海藻酸钠）的铜绿假单胞菌 KBN12 有氧培养 9 天可将 BB 染料脱色 87.67%。对 BB 染料脱色后的化学结构进行傅里叶变换红外光谱（FTIR）和气相色谱（GC）分析，发现功能和化学基团发生了变化。毒性和土壤呼吸酶测定表明，脱色染料或染料产物的毒性比纯 BB 染料低。
铜绿假单胞菌 KBN12 的包埋被证明是一种有效的 BB 染料脱色或修复方法。
