Department of Biosciences, Veer Narmad South Gujarat University. Surat, Gujarat, India.
Arch Microbiol. 2021 Jul;203(5):2669-2680. doi: 10.1007/s00203-021-02258-x. Epub 2021 Mar 13.
The optimization of the bacterium Pseudomonas stutzeri SPM-1, obtained from textile wastewater dumping sites of Surat, Gujarat was studied for the degradation of the textile azo dye Procion Red-H3B. The strain showed significant activities of azoreductase (95%), laccase (76%) and NADH-DCIP reductase (88%) at 12, 10 and 8 h of growth, respectively, indicating the evidence for reductive cleavage of the dye. The optimization was carried on phenanthrene enrichment medium followed by exposing it to variable environmental factors and nutritional sources. The complete decolourization of dye (50 mg/L) happened within 20 h of incubation at pH 8 and temperature 32 ± 0.2 °C under microaerophilic condition. Decolourization was monitored with the shifting of absorbance peak in UV-Vis spectrophotometry and HPLC analysis. The changes in the functional groups were confirmed by the presence of new peaks in FT-IR data. GC-MS analysis helped in recognizing the degraded dye compounds thus elucidating the proposed pathway for Procion Red-H3B. The potential of bioremediation process was completed by phytotoxicity test using two plants Vigna radiata and Cicer arietinum. Our study concludes that the strain Pseudomonas stutzeri SPM-1, with its rapid decolourization efficiency holds noteworthy prospective in industrial application for textile wastewater treatment.
从古吉拉特邦苏拉特的纺织废水倾倒场获得的假单胞菌 SPM-1 的优化，用于降解纺织偶氮染料 Procion Red-H3B。该菌株在生长 12、10 和 8 小时时分别显示出显著的偶氮还原酶（95%）、漆酶（76%）和 NADH-DCIP 还原酶（88%）活性，表明染料具有还原裂解的证据。通过菲恩坦恩富集培养基进行了优化，然后将其暴露于可变环境因素和营养源中。在微需氧条件下，pH 值为 8，温度为 32±0.2°C 时，孵育 20 小时即可完全脱色（50mg/L）。通过 UV-Vis 分光光度法和 HPLC 分析监测脱色情况，吸收峰的移动。FT-IR 数据中存在新峰，证实了功能基团的变化。GC-MS 分析有助于识别降解的染料化合物，从而阐明了 Procion Red-H3B 的建议途径。利用两种植物豇豆和鹰嘴豆进行植物毒性试验完成了生物修复过程的潜力。我们的研究结论是，具有快速脱色效率的假单胞菌 SPM-1 菌株在纺织废水处理的工业应用中具有重要的前景。
